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RNA Sequencing

Introduction to RNA Sequencing

RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing researchers with visibility into previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions, and across a broad range of other study designs.

RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, and other features without the limitation of prior knowledge.

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Propelling Progress with RNA-Seq

RNA sequencing can have far-reaching effects on research and innovation, transforming our understanding of the world around us.

Benefits of RNA Sequencing

RNA-Seq with next-generation sequencing (NGS) is increasingly the method of choice for researchers studying the transcriptome. It offers numerous advantages over gene expression arrays.

  • Broader dynamic range enables more sensitive and accurate measurement of gene expression
  • Not limited by prior knowledge - captures both known and novel features
  • Can be applied to any species, even if reference sequencing is not available
  • A better value, often delivering advantages at a comparable or lower price per sample than many arrays
RNA Sequencing Empowers Transcriptomics

Learn how RNA-Seq is advancing transcriptome research in various fields, and how gene regulation studies can provide complementary information.

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RNA Sequencing Publication Review

RNA-Seq is by far the most cited NGS method. This collection contains protocol diagrams, advantages and disadvantages, and related peer-reviewed publications on various RNA-Seq methods featuring Illumina technology.

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RNA Sequencing Publication Review
New To NGS?

Find out how Illumina NGS technology works and what types of experiments it enables.

How Can I Use NGS to Analyze RNA?

Learn about 7 key RNA-Seq methods. Find out how they differ to help you determine the method most appropriate for your research.

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How Can I Apply RNA-Seq?

Study gene expression and transcriptome changes with cancer RNA-Seq.

Analyze host-pathogen interactions or bacterial transcriptome signatures with microbial RNA-Seq.

Study drug response RNA biomarkers.

Frequently Purchased Together

Featured RNA Sequencing Articles

Precision Immunotherapies Using Tumor-Specific HLA Ligands
Precision Immunotherapies Using Tumor-Specific HLA Ligands

RNA-Seq and HLA typing are increasing the power and efficiency of a target discovery platform.

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The Time Is Now for Microbiome Studies
The Time Is Now for Microbiome Studies

Transcriptomics and whole-genome shotgun sequencing provide researchers and pharmaceutical companies with data to refine drug discovery and development.

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RNA Sequencing Considerations
RNA Sequencing Considerations

Learn about read length and depth requirements for RNA-Seq and find resources to help with experimental design.

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Library Prep for RNA Sequencing

Library Prep for RNA Sequencing

Advances in RNA-Seq library prep are revolutionizing the study of the transcriptome. Our enhanced RNA-Seq library prep portfolio spans multiple types of sequencing studies. These solutions offer rapid turnaround time, broad study flexibility, and sequencing scalability.

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Create Custom RNA-Seq Protocols

A fast, flexible, and mobile-friendly tool, our Custom Protocol Selector helps you generate RNA sequencing protocols tailored to your experiment.

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Create Your NGS Protocol

Featured Products

Illumina Stranded mRNA Prep
Illumina Stranded mRNA Prep

A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard (non-degraded) RNA.

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NextSeq 1000 & 2000 Systems
NextSeq 1000 & 2000 Systems

These cost-efficient, user-friendly, mid-throughput benchtop sequencers offer extreme flexibility to support new and emerging applications.

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DRAGEN RNA Pipeline
DRAGEN RNA Pipeline

Performs alignment, quantification, and fusion detection.

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RNA-Seq Differential Expression
RNA-Seq Differential Expression

Enables differential gene expression analysis.

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RNA-Seq Library Prep Product Portfolio

Enhanced RNA-Seq Library Prep Portfolio

The new enhancements deliver solutions for studying RNA that provide rapid turnaround time, broad study flexibility and sequencing scalability, while delivering exceptional data quality for infectious disease, oncology and genetic disease research.

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Additional Resources

Buyer’s Guide: Simple RNA-Seq Workflows
Buyer’s Guide: Simple RNA-Seq Workflows

Evaluating options for next-generation RNA sequencing.

Deeper Insights with RNA-Seq
Deeper Insights with RNA-Seq

RNA sequencing provides deeper insights for complex research. See how RNA-Seq is helping this lab move beyond gene expression.

Gene Target Identification
Gene Target Identification

Uncover gene targets and pathways tied to disease.

FAQs: RNA-Seq Data Analysis
FAQs: RNA-Seq Data Analysis

Demystifying data analysis with answers to some of the most common frequently asked questions.