AmpliSeq for Illumina Comprehensive Cancer Panel

Targeted panel investigating the exonic regions of 409 genes with known associations to cancer.Read More...
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Panel

AmpliSeq™ Comprehensive Cancer Panel for Illumina®

20019160

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ CD Indexes Set A for Illumina® (96 Indexes, 96 Samples)

20019105

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Accessory Products

AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Comprehensive Cancer Panel provides a targeted resequencing solution for analyzing somatic mutations across 409 genes with known associations to cancer. Key features include:

Relevant Gene Content
  • Target all-exon coverage of 409 cancer-associated genes
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations below 5% frequency using local or cloud analysis

The Comprehensive Cancer Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This panel detects variants within multiple cancer types, including lung, colon, breast, ovarian, melanoma prostate, and hematologic malignancies. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System Samples per run: mid output: 4, high output: 12 (assumes minimum coverage of 500×) 2 × 150 bp

Product Comparison

AmpliSeq for Illumina Comprehensive Cancer Panel AmpliSeq for Illumina Cancer Hotspot Panel v2
Assay Time 6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time)
Cancer Type Pan-Cancer, Solid Tumor Pan-Cancer, Solid Tumor
Content Specifications Full exon coverage of 409 genes associated with cancer. Hotspot regions of 50 genes with known associations to cancer
Description Somatic analysis research across pan-cancer genes. Somatic analysis research into hotspot regions of 50 cancer-related genes.
Hands-On Time < 1.5 hours < 1.5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool)
Method Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing 96 dual index combinations 96 dual index combinations
Specialized Sample Types Blood, FFPE Tissue Blood, FFPE Tissue
Species Category Human Human

Method-Specific Workflow Example

 

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