Beyond conventional cell and molecular biology research methods

NGS methods enable global analyses of gene expression and regulation

Cellular & Molecular Biology Research

Traditionally, cell and molecular biology research seeks to understand the function of a single gene, gene family, or signal transduction pathway, often through the disruption or modification of single genes. Researchers use signal-based methods such as microscopy, flow cytometry, and protein blotting and molecular assays such as PCR/qPCR, Sanger sequencing, and gene expression arrays. This approach is time-consuming and does not always result in conclusive findings.

Array and next-generation sequencing (NGS) technologies from Illumina can broaden cell and molecular biology research beyond the conventional methods of protein-interaction and single-gene functional studies. Illumina technology enables analysis across the genome, transcriptome, and epigenome. Results can inform experimental design and subsequent studies, saving time and allowing researchers to publish sooner.

NGS in Cell Biology Research

See how NGS offers new methods for expanding cell and molecular biology research.

Read Introduction

Compare the benefits of NGS technology to qPCR and learn which one to choose.

Learn More

Genetic Studies of Memory

Dr Matt Huentelman uses single-cell RNA sequencing to study the genetic and neurological variables affecting learning and memory.

Learn More
Single-Cell RNA Sequencing

Providing a high-resolution view of cell-to-cell variation for cellular and molecular biology research. This method allows you to explore the distinct biology of individual cells within a complex tissue, and understand subpopulation responses to environmental cues.

Learn More
/content/dam/us/en/assembled-assets/web-graphic/targeting-batch27-web-graphic/single-cell-rna-sequencing.jpg

SureCell Whole Transcriptome Analysis 3' Library Prep Kit: This kit supports transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.

NextSeq 550 System: This system combines sequencing and array scanning on a single platform.

BaseSpace Sequence Hub Single-Cell RNA-Seq App: Designed for use with the SureCell WTA 3' Library Prep Kit, this app performs cell and gene counting, filtering, and reporting.

AmpliSeq for Illumina Targeted Resequencing Solution:A streamlined, scalable amplicon sequencing solution, producing high-confidence data from low-input DNA and RNA samples.

Infinium Global Screening Array: A next-generation genotyping array for population-scale genetics, variant screening, pharmacogenomics studies, and precision medicine research.

MethylationEPIC BeadChip: Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.

From Genotype to Phenotype

Dr Lachlan Jolly discusses insights into gene function in cell models of brain development.

View Webinar
Interested in receiving newsletters, case studies, and information on complex disease genomics? Please enter your email address.
The Singular Neuron Understanding Complex Biology Once Cell at a Time
The Singular Neuron Understanding Complex Biology Once Cell at a Time

Learn how single-cell RNA sequencing can be used in-vivo to understand how individual cells function in their microenvironment.

View Video
Chan Zuckerberg Biohub and the NovaSeq System
Chan Zuckerberg Biohub and the NovaSeq System

The Chan Zuckerberg Biohub uses the NovaSeq System to conduct innovative experiments in genomics.

View Video
Cellular Research Review
Cellular Research Review

This review highlights recent publications that used Illumina technology for single-cell sequencing.

Read Review
Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells
Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells

Gain insight into how individual cells contribute to the function of a complex tissue such as peripheral blood.

Read App Note
Simple, Customized RNA-Seq Workflows
Simple, Customized RNA-Seq Workflows

Evaluating RNA sequencing options for cellular and molecular biology research including single-cell RNA sequencing

Download Guide
Software Tools to Simplify Data Analysis
Software Tools to Simplify Data Analysis

Illumina bioinformatics tools can help researchers manage, analyze, and interpret genomic data.

Explore Bioinformatics Software
Mapping Neural Diversity with Single-Cell RNA-Seq
Mapping Neural Diversity with Single-Cell RNA-Seq

The ability to analyze gene expression signatures from individual cells is transforming the way neurons are classified.

Read Interview
Single-Cell Sequencing on the NovaSeq 6000 System
Single-Cell Sequencing on the NovaSeq 6000 System

This scalable, robust, single-cell NGS methodology enables routine transcriptome profiling at single-cell resolution.

Read App Note