Beyond conventional cell and molecular biology research methods

NGS methods enable global analyses of gene expression and regulation

Cellular & Molecular Biology Research

Traditionally, cell and molecular biology research seeks to understand the function of a single gene, gene family, or signal transduction pathway, often through the disruption or modification of single genes. Researchers use signal-based methods such as microscopy, flow cytometry, and protein blotting and molecular assays such as PCR/qPCR, Sanger sequencing, and gene expression arrays. This approach is time-consuming and does not always result in conclusive findings.

Array and next-generation sequencing (NGS) technologies from Illumina can broaden cell and molecular biology research beyond the conventional methods of protein-interaction and single-gene functional studies. Illumina technology enables analysis across the genome, transcriptome, and epigenome. Results can inform experimental design and subsequent studies, saving time and allowing researchers to publish sooner.

NGS in Cell Biology Research

See how NGS opens new avenues to exploring and understanding the cellular activity of disease.

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Compare the benefits of NGS technology to quantitative PCR and find out which one to choose.

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Genetic Studies of Memory

Translational Genomics Research Institute (TGen) researchers use single-cell RNA sequencing to study the genetic and neurological variables affecting learning and memory.

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Single-Cell RNA Sequencing

Explore the distinct biology of individual cells within a complex tissue, or investigate the molecular mechanisms behind subpopulation responses to environmental cues.

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Targeted Resequencing

Focus on sequencing targeted genomic regions of interest, or simultaneously sequence large numbers of genes associated with a disease or phenotype.

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Methylation Analysis

Quantitatively investigate methylation patterns using sequencing- and array-based techniques, and gain valuable insight into gene regulation.

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Genotyping

Explore genetic variants such as single nucleotide polymorphisms (SNPs) and large structural changes in DNA  using genome-wide or targeted approaches.

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Genome Editing

Find out how NGS enables researchers to confirm CRISPR gene knockouts, analyze off-target effects, assess the the functional impact of edits, and more.

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Beginner's Guide to Next-Generation Sequencing

Considering bringing next-generation sequencing to your lab, but unsure where to start? These resources cover key topics in NGS and are designed to help you plan your first experiment.

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Beginner's Guide to Next-Generation Sequencing

SureCell Whole Transcriptome Analysis 3' Library Prep Kit: This kit supports transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.

NextSeq 550 System: This system combines sequencing and array scanning on a single platform.

BaseSpace Sequence Hub Single-Cell RNA-Seq App: Designed for use with the SureCell WTA 3' Library Prep Kit, this app performs cell and gene counting, filtering, and reporting.

AmpliSeq for Illumina Targeted Resequencing Solution: A streamlined, scalable amplicon sequencing solution, producing high-confidence data from low-input DNA and RNA samples.

Infinium Global Screening Array: A next-generation genotyping array for population-scale genetics, variant screening, pharmacogenomics studies, and precision medicine research.

MethylationEPIC BeadChip: Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.

From Genotype to Phenotype

Dr Lachlan Jolly discusses insights into gene function in cell models of brain development.

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The Singular Neuron - Understanding Complex Biology One Cell at a Time
The Singular Neuron - Understanding Complex Biology One Cell at a Time

Learn how single-cell RNA sequencing can be used to understand how individual cells function in their microenvironment.

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Chan Zuckerberg Biohub and the NovaSeq System
Chan Zuckerberg Biohub and the NovaSeq System

The Chan Zuckerberg Biohub uses the NovaSeq System to conduct innovative experiments in genomics.

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Cellular Research Review
Cellular Research Review

This review highlights recent publications that used Illumina technology for single-cell sequencing.

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Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells
Single-Cell RNA Sequencing of Peripheral Blood Mononuclear Cells

Gain insight into how individual cells contribute to the function of a complex tissue such as peripheral blood.

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Simple, Customized RNA-Seq Workflows
Simple, Customized RNA-Seq Workflows

Evaluating RNA sequencing options for cellular and molecular biology research including single-cell RNA sequencing

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Software Tools to Simplify Data Analysis
Software Tools to Simplify Data Analysis

Illumina bioinformatics tools can help researchers manage, analyze, and interpret genomic data.

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Mapping Neural Diversity with Single-Cell RNA-Seq
Mapping Neural Diversity with Single-Cell RNA-Seq

The ability to analyze gene expression signatures from individual cells is transforming the way neurons are classified.

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Single-Cell Sequencing on the NovaSeq 6000 System
Single-Cell Sequencing on the NovaSeq 6000 System

This scalable, robust, single-cell NGS methodology enables routine transcriptome profiling at single-cell resolution.

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