With the Infinium MethylationEPIC BeadChip Kit, researchers can interrogate over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution. Multiple samples, including FFPE, can be analyzed in parallel to deliver high-throughput power while minimizing the cost per sample.
Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods. The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues.
Comprehensive Genome-Wide Coverage
This kit includes content categories requested by methylation experts, such as:
- CpG sites outside of CpG islands
- Non-CpG methylated sites identified in human stem cells (CHH sites)
- Differentially methylated sites identified in tumor versus normal
- FANTOM5 enhancers
- ENCODE open chromatin and enhancers
- DNase hypersensitive sites
- miRNA promoter regions
Integrated Analysis Software
MethylationEPIC data analysis is supported by the GenomeStudio Methylation Module. GenomeStudio Software enables researchers to effortlessly perform differential methylation analysis for small-scale studies. It features advanced visualization tools that enable researchers to view vast amounts of data in a single graph, such as heat maps, scatter plots, and line plots.
These tools and the GenomeStudio Genome Browser display valuable information such as chromosomal coordinates, percent GC, location in a CpG Island, and methylation β values.
For large-scale studies, there are many free analysis packages that work in the software framework R for normalization, and differential analysis of methylation data. Instructional videos explain the use of these data analysis packages.