Infinium MethylationEPIC Kit

With the Infinium MethylationEPIC BeadChip Kit, researchers can interrogate over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution. Multiple samples, including FFPE, can be analyzed in parallel to deliver high-throughput power while minimizing the cost per sample.

Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods. The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues.

Comprehensive Genome-Wide Coverage

This kit includes content categories requested by methylation experts, such as:

• CpG sites outside of CpG islands
• Non-CpG methylated sites identified in human stem cells (CHH sites)
• Differentially methylated sites identified in tumor versus normal
• FANTOM5 enhancers
• ENCODE open chromatin and enhancers
• DNase hypersensitive sites
• miRNA promoter regions

Integrated Analysis Software

MethylationEPIC data analysis is supported by the GenomeStudio Methylation Module. GenomeStudio Software enables researchers to effortlessly perform differential methylation analysis for small-scale studies. It features advanced visualization tools that enable researchers to view vast amounts of data in a single graph, such as heat maps, scatter plots, and line plots.

These tools and the GenomeStudio Genome Browser display valuable information such as chromosomal coordinates, percent GC, location in a CpG Island, and methylation β values.

For large-scale studies, there are many free analysis packages that work in the software framework R for normalization, and differential analysis of methylation data. Instructional videos explain the use of these data analysis packages.