Infinium MethylationEPIC v2.0 Kit

Robust methylation profiling microarray providing extensive coverage of CpG islands, genes, and enhancers for epigenome-wide association studies.

> 1000 samples per week (per Tecan for 8 sample BeadChip)

Sample throughput

8 samples per array

Number of samples

>  935K

Number of markers

See full details in the specifications table

Overview

The Infinium MethylationEPIC v2.0 kit is a genome-wide methylation screening tool targeting > 935K CpG sites in the most biologically significant regions of the human methylome.

  • Quantitative interrogation of CpGs at single-nucleotide resolution, with accurate and precise methylation measurements independent of read depth

  • Primary analysis and quality control with the GenomeStudio Methylation Module

  • Updated content guided by expert evaluations of EPIC v1.0

Cost-effective screening

A comparatively low total cost per sample and scalability enables labs to screen large cohorts efficiently.

Trusted by epigenetics researchers

Illumina methylation BeadChips have powered > 10 years of groundbreaking research, with most published epigenome-wide association studies (EWAS) to date completed on MethylationEPIC or its predecessor. Harness methylation data in data repositories like the Gene Expression Omnibus (GEO) or The Cancer Genome Atlas (TCGA).

Compatible with multiple sample types

The assay is FFPE-compatible, enabling studies to be completed on large biorepositories of tumors. It is also compatible with fresh/frozen tissue, whole blood, buccal cells, cfDNA, saliva, and other sample types.


Specifications


Required products

Requires purchase of a kit that matches the number of samples you run at a time. Please note that each kit is processed as a single batch and is not designed to be divided across multiple experiments. If you are processing several low-throughput batches, order multiple small kits. The kits contain all required reagents for performing methylation screening, except for the bisulfite conversion kits, which are optional products that can be purchased separately. 

/ Results

Applications

Infinium MethylationEPIC v2.0 BeadChip is a robust microarray that enables discovery of methylation-based biomarkers for research applications in cancer, genetic diseases, aging, and molecular epidemiology.

Example workflow

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Infinium MethylationEPIC v2.0 Kit



Related applications and methods

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Infinium MethylationEPIC v2.0 Kit Infinium Custom Methylation Kit Infinium Mouse Methylation BeadChip
Assay type Infinium HD Methylation Infinium HD Methylation
Automation capability Automated array loader Automated array loader Automated array loader
Input quantity 250 ng DNA 250 ng DNA
Instruments NextSeq 550 System, NextSeq 550Dx in Research Mode, iScan System iScan System iScan System
Method Methylation array Methylation array Methylation array
Nucleic acid type DNA DNA DNA
Number of markers

>  935K

Fixed markers: 285k

Number of samples 8 samples per array 12 samples per array
Sample throughput

> 1000 samples per week (per Tecan for 8 sample BeadChip)

Sample type details

Also compatible with buccal cells and fresh/frozen tissue

May be used with fresh/frozen tissue. Analyze changes in DNA methylation induced by exogenous chemicals, including DAC.

Specialized sample types Blood, FFPE Tissue, Cell-Free DNA, Saliva Blood, Cell-Free DNA, Saliva Blood, FFPE Tissue, Saliva
Species category Human Mouse, Human Mouse
Technology Microarray Microarray Microarray
Variant class Differentially methylated cytosines Differentially methylated cytosines Differentially methylated cytosines

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Infinium MethylationEPIC v2.0 coverage of genomic regions

Infinium MethylationEPIC v2.0 coverage of genomic regions

New and enhanced content enables future epigenetic discoveries.

Dense coverage of CpG islands

Dense coverage of CpG islands

CpG islands and exons that were covered insufficiently on the Infinium MethylationEPIC v1.0 BeadChip are covered thoroughly with additional probes.

Robust data with FFPE samples

Robust data with FFPE samples

FFPE tissue samples show robust performance with a modified version of the Infinium Methylation HD Assay. For precious samples and optimal sample integrity, the Infinium FFPE QC and DNA Restoration Kits are recommended.

High backwards compatibility with previous BeadChips

High backwards compatibility with previous BeadChips

The Infinium MethylationEPIC v2.0 BeadChip builds upon the existing CpG backbones of the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChips.

EPIC v2.0 kits (4)

Inf MethylationEPIC V2.0 Kit (8 Spl)

20087706

Includes one, 8-sample BeadChip and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 8 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.

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Inf MethylationEPIC V2.0 Kit (16 Spl)

20087707

Includes two, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 16 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.

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Inf MethylationEPIC V2.0 Kit (32 Spl)

20087708

Includes four, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 32 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.

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Inf MethylationEPIC V2.0 Kit (96 Spl)

20087709

Includes 12, 8-sample BeadChips and reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 96 DNA samples. Requires 250 ng DNA for input. Each kit is processed as a single batch. Purchase bisulfite conversion kits separately.

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EPIC v2.0 semi-custom kits (3)

Inf MethylationEPIC-8+ v2.0 Kit (16 Spl)

20097750

Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 2 beadchips with reagents for processing 16 samples.

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Inf MethylationEPIC-8+ v2.0 Kit (32 Spl)

20097751

Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 4 beadchips with reagents for processing 32 samples.

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Inf MethylationEPIC-8+ v2.0 Kit (96 Spl)

20097752

Semi-custom EPIC v2 containing a custom selection of 3,000-10,000 attempted beadtypes on top of EPIC v2. Kit contains 12 beadchips with reagents for processing 96 samples.

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Services (1)

Infinium® Assay: An Introduction – Customer Site

20015273

Three-day, hands-on instruction at customer site to familiarize users with the essential steps in the Infinium protocol. Course provides hands-on training in sample and BeadChip preparation, sample scanning using the iScan System, and primary data evaluation and analysis using array analysis software.

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FAQs

The Infinium Methylation EPIC v2.0 BeadChip Kit leverages powerful Infinium chemistry to detect cytosine methylation at CpG islands based on highly multiplexed genotyping of bisulfite-converted genomic DNA. Learn more about quantitative array-based methylation measurement.

This kit uses the Illumina Methylation Assay, a microarray technology that provides quantitative methylation measurement at the single-CpG-site level, powering research that examines the biological role of DNA methylation in normal and diseased cells. Learn more about the Illumina Methylation Assay.

The Infinium MethylationEPIC v2.0 BeadChip Kit contains 186K new probes that target known enhancers, super-enhancers, CTCF-binding sites, and open regions of chromatin associated with primary tumors identified by ATAC-Seq and ChIP-seq experiments. Learn about additional improvements in the Infinium MethylationEPIC v2.0 data sheet.

The GenomeStudio Methylation Module extracts beta values and provides clustering and normalization for Infinium MethylationEPIC array data. In addition, there are many freeware applications in Bioconductor, such as chAMP and SeSAMe, that provide enhanced normalization and visualization options. Illumina does not provide technical support for these freeware solutions directly. See more methylation array data analysis tips.

The Zymo EZ DNA Methylation Lightning Kit is the recommended bisulfite conversion kit for the Infinium workflow. Third party bisulfite conversion kits must be purchased separately. Learn more about validated bisulfite conversion kits for Infinium Methylation BeadChips.

The output file is an average of ~2.75 Gb with JPEG files, which is the default setting.

The scan takes ~18–37 minutes per BeadChip.

Yes, the Infinium MethylationEPIC v1.0 and HumanMethylation450 BeadChip kits have been discontinued. The Infinium MethylationEPIC v2.0 Kit, built upon the same reliable Infinium chemistry foundation, offers enhanced, expert-selected content and is the recommended replacement for both kits. 

/ Results

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