Understanding the role of methylation in regulating gene expression

DNA Methylation Analysis

DNA methylation plays an important role in regulating gene expression. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction. DNA methylation is also a common subject of agrigenomic investigations into responses to drought, temperature extremes, and other environmental changes.

High-throughput technologies such as next-generation sequencing (NGS) and microarrays enable researchers to perform genome-wide methylation profiling. These technologies offer new ways to understand the significance of DNA methylation, providing novel insights into the functional consequences of variation.

 
Methylation Sequencing with NGS

NGS enables comprehensive profiling of methylation patterns at single-base resolution across the entire genome, for the highest clarity in epigenetic studies.

Methylation Arrays

Arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

Most important to me Complete methylome coverage High throughput/large sample number
Least important to me Throughput Coverage
#CpGs covered > 36 million 850,000
Species All Human
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Epigenetic Changes in Cancer
Epigenetic Changes in Cancer

Studies of epigenetic alterations in cancer, such as aberrant methylation and transcription factor binding, can provide insight into important tumorigenic pathways. As altered methylation often activates or silences genes, changes in the epigenome can affect gene expression and the rate of cancer progression. Learn more about cancer epigenetics.

Methylation Profiling for Complex Disease
Methylation Profiling for Complex Disease

Genome-wide methylation profiling can help researchers understand the functional mechanisms at work in complex disease cases. Aberrant cytosine methylation can impact gene expression, and has been implicated in many disease processes, such as Alzheimer’s disease and asthma. Learn more about complex disease research.

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Field Guide to Methylation Methods
Field Guide to Methylation Methods

An overview of the impact of cytosine methylation and the primary methods used to detect cytosine modifications.

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When Whole-Genome Sequencing Is Not Enough
When Whole-Genome Sequencing Is Not Enough

Researchers explore cancer subtypes using Illumina technology for whole-exome, whole-genome, and whole-epigenome sequencing.

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Epigenome Roadmap
Epigenome Roadmap

A collection of research papers that systematically characterize epigenomic landscapes in primary human tissues and cells.

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Epigenome Blueprint
Epigenome Blueprint

A consortium generating 100 reference epigenomes to understand gene activation and repression in healthy and diseased human cells.

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Whole-Genome Methylation Analysis
Whole-Genome Methylation Analysis

TruSeq DNA Methylation rapidly produces whole-genome bisulfite sequencing libraries. Pre-library bisulfite conversion captures full sample diversity.

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Methylation and Metastasis
Methylation and Metastasis

Researchers investigate the epigenetics behind cancer metastasis using DNA methylation microarrays and NGS.

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