AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run.
Combined with Illumina next-generation sequencing (NGS) technology, AmpliSeq for Illumina sequencing offers high-confidence data to researchers in a wide variety of application areas, including cancer and genetic diseases. AmpliSeq for Illumina works with DNA and RNA samples and requires as little as 1 ng of input.
AmpliSeq panels can accommodate high-quality samples such as blood, cell culture, or fresh frozen tissue and also challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue. Select from predesigned panels or customize your content for a variety of genomes and unmatched flexibility.
AmpliSeq for Illumina products are only sold by Illumina and the complete workflow, from design to analysis, is supported by Illumina.
Sequence with innovative NGS platforms that deliver exceptional data quality and accuracy, at any scale, with low hands-on time:
AmpliSeq for Illumina is compatible with samples where available input quantity and quality are not limiting, such as blood, cell culture, or fresh-frozen tissues as well as challenging sample types, such as formalin-fixed paraffin-embedded (FFPE) tissue. The input requirement for DNA and RNA ranges from 1 ng to 100 ng, depending on the application needs. For most applications, we recommend 10 ng input per pool.
Library preparation with AmpliSeq for Illumina is both fast and simple. Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.
AmpliSeq chemistry allows researchers to analyze hundreds of genes simultaneously with ultra-high multiplexed PCR with 12 to more than 24,000 amplicons in a single panel. Achieve complete coverage of large targets using multiple primer pools to create overlapping amplicons.
Learn how this addition to the Illumina library prep portfolio provides a simple, fast, and robust sequencing option to achieve high-confidence data from even low-quality DNA and RNA samples. This video highlights the ease and simplicity of each step of the workflow, from design to analysis.View Video
5-7 hours of total time
1.5 hours hands-on
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