AmpliSeq for Illumina – a comprehensive targeted resequencing solution

Fast workflow and robust performance from a few to hundreds of genes, with as little as 1 ng input

Hear How Our Partnership Can Help Your Lab

AmpliSeq for Illumina is a suite of AmpliSeq chemistry products that are compatible with Illumina next-generation sequencing platforms. The AmpliSeq for Illumina solution offers a highly multiplexed polymerase chain reaction (PCR)-based workflow for use with targets ranging from a few to hundreds in a single run.

Combined with Illumina next-generation sequencing (NGS) technology, AmpliSeq for Illumina sequencing offers high-confidence data to researchers in a wide variety of application areas, including cancer and genetic diseases. AmpliSeq for Illumina works with DNA and RNA samples and requires as little as 1 ng of input.

AmpliSeq panels can accommodate high-quality samples such as blood, cell culture, or fresh frozen tissue and also challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue. Select from predesigned panels or customize your content for a variety of genomes and unmatched flexibility.

AmpliSeq for Illumina products are only sold by Illumina and the complete workflow, from design to analysis, is supported by Illumina.

Introducing Ampliseq
Introducing AmpliSeq for Illumina

Learn how AmpliSeq for Illumina provides researchers with a high-confidence solution to detect variants with flexible input ranges and sample types.

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Expand Your Research Capabilities

Sequence with innovative NGS platforms that deliver exceptional data quality and accuracy, at any scale, with low hands-on time:

  • Target variants for many applications with ready-to-use or custom panels
  • Use a multiplexed, PCR-based workflow
  • Transform data into insight with a variety of data analysis solutions
Sequence a Broad Range of Samples

AmpliSeq for Illumina is compatible with samples where available input quantity and quality are not limiting, such as blood, cell culture, or fresh-frozen tissues as well as challenging sample types, such as formalin-fixed paraffin-embedded (FFPE) tissue. The input requirement for DNA and RNA ranges from 1 ng to 100 ng, depending on the application needs. For most applications, we recommend 10 ng input per pool.

Prepare Libraries Quickly and Easily

Library preparation with AmpliSeq for Illumina is both fast and simple. Prepare an on-target, high-uniformity amplified library in as little as 5 hours with just 1.5 hours of hands-on time.

Analyze Hundreds of Genes at Once

AmpliSeq chemistry allows researchers to analyze hundreds of genes simultaneously with ultra-high multiplexed PCR with 12 to more than 24,000 amplicons in a single panel. Achieve complete coverage of large targets using multiple primer pools to create overlapping amplicons.

Quality and Ease of Use

Sequencing Amplified: AmpliSeq for Illumina

Learn how this addition to the Illumina library prep portfolio provides a simple, fast, and robust sequencing option to achieve high-confidence data from even low-quality DNA and RNA samples. This video highlights the ease and simplicity of each step of the workflow, from design to analysis.

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AmpliSeq for Illumina Sequencing Solution

Prepare high-quality libraries quickly and simply using a multiplexed, PCR-based workflow
AmpliSeq for Illumina Targeted Resequencing Solution

A streamlined, scalable amplicon sequencing solution, producing high-confidence data from low-input DNA and RNA samples.

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Accurate Data Across Various Sample Types

AmpliSeq for Illumina Focus Panel allows oncology researchers to target hotspots, single nucleotide polymorphisms (SNVs), indels, copy number variation (CNVs), and gene fusions from DNA or RNA in a single workflow.

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AmpliSeq for Illumina FAQs

Wondering how the various AmpliSeq panels for Illumina sequencing differ? Have questions about which components you’ll need, panel customization and post-sequencing analysis options, or where to go for technical support?

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Clinical Cancer Research

Our clinical cancer research solutions deliver accurate genomic information, and enable labs to analyze multiple genes in a single test.

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