The field of cultivation-independent microbiome analysis has developed rapidly and is at the threshold of major advances in read-length, accuracy and rigor through application of standards. This webcast will discuss new sequencing methods, including those generating synthetic long reads, with a focus on their relevance for microbial community analysis.
However, difficulties associated with low biomass – particularly background contamination – and with high non-microbial host nucleic acids, remain. The speaker will address practical approaches to high host and low microbial biomass issues, fundamental concerns related to PCR-amplification bias, and how to transition to alternate approaches for analyzing complex microbial communities.
Dr. Stefan J. Green
RRC - Genome Research Division University of Illinois at Chicago
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