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Nextera Flex: A Robust and Self-Normalizing Enzymatic Library Prep Technology

Library preparation for Illumina sequencing consists of DNA fragmentation and adapter-tagging. DNA fragmentation can be achieved using enzymatic or mechanical methods. Enzymatic methods are typically more convenient but can create challenges around sensitivity to DNA quantitation, and/or require extensive optimization to achieve robustness.  

Nextera Flex is a novel bead-linked-transposome technology, which addresses these shortcomings whilst retaining the convenience of enzymatic fragmentation. Nextera Flex is compatible with a wide range of DNA inputs, providing new levels of robustness while minimizing the requirement for quantitation post-library prep for an input range of 100-500ng for whole genome sequencing and 50-1000ng for targeted enrichment. In addition, the underlying technology will normalize the libraries to give a consistent yield and insert size from a range of DNA inputs. When paired with the Nextera Flex lysis kit, customers can employ a quantification-free workflow to enable normalized library creation from raw samples such as blood and saliva. This new technology offers customers a novel, easy-to-use, fast, and simple solution for library preparation. 

Andrew Slatter and Joshua Burgess,Illumina
Topic: Agriculture Genomics : Cancer Genomics : Reproductive & Genetic Health : Human Genetics & Genetic Disease : Microbiology & Infectious Disease : Sequencing : Services