ScriptSeq Library Prep Kit Selection Table

ScriptSeq Kit Selection Guide

Fast Preparation

Fast, 1-day RNA-Seq library prep procedure

Directionality

Directional (stranded) RNA-Seq libraries

Flexible Sequencing

Libraries for single-read, multiplex or paired-end sequencing on Illumina MiSeq, HiSeq and GAII sequencers

FFPE Compatibility

Use with intact, degraded, and FFPE RNA

Sample Type Kit Input RNA Additional Comments
Poly(A)-selected or rRNA-depleted RNA (provided by the user) from any source including FFPE tissue ScriptSeq v2 RNA-Seq Library Preparation Kit 500 pg - 50 ng of poly(A)-selected or rRNA-depleted RNA (provided by the user) Requires use of FailSafe PCR Enzyme Mix (provided by the user) This kit does not include a Ribo-Zero rRNA or a Globin-Zero globin mRNA removal kit
Human/Mouse/Rat and other higher vertebrates; FFPE RNA ScriptSeq Complete Gold Kit (Human/Mouse/Rat) 1 - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
Blood ScriptSeq Complete Gold Kit (Blood) 1 - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Globin-Zero rRNA/globin mRNA removal kit and ScriptSeq Library Prep kit
Microbiome, bacterial infected human/mouse/rat ScriptSeq Complete Gold Kit (Epidemiology) 500 ng - 2.5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
Bacteria ScriptSeq Complete Kit (Bacteria) 1 - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
Plant leaf ScriptSeq Complete Kit (Plant Leaf) 1 - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
Plant seed or root ScriptSeq Complete Kit (Plant Seed/Root) 1 - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
Yeast ScriptSeq Complete Gold Kit (Yeast) 100 ng - 5 µg of total RNA Requires use of FailSafe PCR Enzyme Mix (provided by the user) Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit
RNA Sequencing

RNA sequencing (RNA-Seq) enables highly sensitive analysis of expression across the transcriptome. Detect novel or known features and quantify RNA activity.

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Library Prep and Array Kit Selector

Determine the best kit for your needs based on your project type, starting material, and method of interest.

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