Science and Education

Bubble-Seq

Bubble-Seq

Bubble-Seq: Libraries of restriction fragments that contain replication initiation sites (bubbles) in vivo.

DNA was biotinylated by 3'-end tailing reaction and Biotin-16-dUTP. Biotinylated DNA was sonicated and captured with streptavidin-coated beads. The free DNA fragments were then purified..

Pros:
  • Simple protocol
Cons:
  • Not widely adopted by the scientific community yet
  1. Foulk M. S., Urban J. M., Casella C. and Gerbi S. A. (2015) Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins. Genome Res 25: 725-735
For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

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