This kit is compatible with unique dual index kits (available soon), which allow multiplexing of up to 96 samples.

TruSeq DNA PCR-Free Library Preparation Kit

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes. Read More...
Select Product(s)

TruSeq DNA PCR-Free LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)



TruSeq DNA PCR-Free LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)



TruSeq DNA PCR-Free HT Library Preparation Kit (96 indexes in plate format, 96 samples)



Product Highlights

TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

  • Shortened gel-free workflows that remove the need for PCR
  • Ability to sequence the most challenging regions
  • Improved genome coverage to identify the greatest number of variants
Sequence the most challenging regions

TruSeq DNA PCR-free kits offer superior coverage of areas which are traditionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants

PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols

Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexible throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

  • TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
  • TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits


Method-Specific Workflow Example


Customer Stories

Feline Social Media Star Gets Sequenced

Researchers from the Max Planck Institute and the University of Pennsylvania crowdfunded the genome sequencing of Lil Bub, using the NextSeq 500 System to uncover the genetic causes of the cat’s rare diseases.

Read More
Single-Cell Transcriptome Sequencing, the Internet, and Memory

TGen researchers are combining crowd-sourcing and single-cell RNA sequencing on HiSeq Systems to adequately power genetic studies of memory.

Read More

Product Comparison

Comparison of TruSeq Library Preparation Kits
Specification TruSeq DNA PCR-Free TruSeq Nano DNA
Kit type LT and HT For NeoPrep System LT and HT
Description Superior genomic coverage with radically reduced library bias and gaps TruSeq Nano DNA workflow performed with the NeoPrep system and reagents Based upon widely adopted TruSeq library prep, with lower input and improved data quality
Workflow LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
Simplified NeoPrep workflow with lower input and less hands-on time LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
Input quantity 1–2 μg 25–75 ng 100–200 ng
Includes PCR No Yes Yes
Assay time ~5 hours 7.5 hours (includes library prep through normalization) 5.5 hours
Hands-on time ~4 hours 30 minutes 4 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp 350 bp or 550 bp
Gel-Free Yes Yes Yes
Number of samples supported 24 (LT) or 96 (HT) samples Up to 16 samples per NeoPrep library card 24 (LT) or 96 (HT)
Size-selection beads Included Included Included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing 24 single indexes or 96 dual-index combinations
Compatible Illumina sequencers MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems

Product Literature

Comparison of TruSeq Library Preparation Kits

Technical Note | PDF(< 1 MB)

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