Illumina DNA PCR-Free Prep is now available. This library prep kit achieves commensurate performance with TruSeq DNA PCR-Free, while reducing turnaround time and hands-on time.

TruSeq DNA PCR-Free

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes. Read More...
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Library Prep

TruSeq DNA PCR-Free Low Throughput Library Prep Kit (24 samples)

20015962

Price
 
 

TruSeq DNA PCR-Free High Throughput Library Prep Kit (96 samples)

20015963

Price
 
 

Index Adapters

IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

Price
 
 

Product Highlights

TruSeq DNA PCR-Free provides simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole genomes. The workflows offer:

  • Shortened gel-free workflows that remove the need for PCR
  • Ability to sequence challenging regions
  • Excellent coverage quality for deep insight into the genome
Sequence challenging regions

TruSeq DNA PCR-Free offers superior coverage of areas that are traditionally difficult to sequence, such as GC-rich regions, promoters, and repetitive content.

The workflows are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Gain deep insight into the genome

PCR-free means reduced library bias and gaps. The result is high data quality and optimal variant detection across the genome. Excellent genome coverage quality means your results have few gaps and good coverage of GC-rich regions.

Save time with a PCR-free protocol

Removing PCR saves time and removes genomic coverage bias associated with PCR steps. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, TruSeq DNA PCR-Free provides a range of enhancements to a widely adopted library preparation workflow.

Access flexible throughput options
  • TruSeq DNA PCR-Free with Single Indexes supports 24-sample manual processing for low-throughput (LT) studies.
  • TruSeq DNA PCR-Free with 96 CD Indexes supports 96-sample processing for high-throughput (HT) studies, and can be automated on liquid-handling robots (or processed manually).
  • IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell.
Fast, Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 100 bp
550 bp insert: ≤ 2 × 150 bp
NovaSeq 6000 System Samples per run (dual flow cell): S1: 8, S2: 16, S4: 48 (based on 30× coverage of a human genome)  350 bp insert: ≤ 2 × 150 bp
550 bp insert: ≤ 2 × 150 bp

Product Comparison

TruSeq DNA PCR-Free Illumina DNA PCR-Free Prep Illumina DNA Prep TruSeq DNA Nano
Assay Time 5 hours total assay time ~1.5 hours ~3-4 hours (from DNA extraction to normalized library) ~6 hours total assay time
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Hands-On Time 4 hours ~45 minutes 1-1.5 hours ~4 hours
Input Quantity 1 ug DNA 25 ng to 300 ng Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). 100 ng genomic DNA
Mechanism of Action Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Bead-linked transposome Bead-linked transposome Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Method Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing De Novo Sequencing, Whole-Genome Sequencing Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing
Multiplexing Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 384-plex Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Specialized Sample Types Not FFPE-Compatible Blood, Low-Input Samples, Not FFPE-Compatible, Saliva Blood, Not FFPE-Validated, Saliva Low-Input Samples, Not FFPE-Compatible
Species Category Human, Mammalian, Mouse, Other, Plant, Rat Any Species, Human Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish Human, Mammalian, Mouse, Other, Plant, Rat
Species Details Compatible with most large DNA genomes. Compatible with any species Compatible with most large DNA genomes.
Target Insert Size 350 bp or 550 bp 450 bp +/- 75 bp ~350bp 350 bp or 550 bp

Method-Specific Workflow Example

 

Customer Stories

Feline Social Media Star Gets Sequenced

Researchers from the Max Planck Institute and the University of Pennsylvania crowdfunded the genome sequencing of Lil Bub, using the NextSeq 500 System to uncover the genetic causes of the cat’s rare diseases.

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