NET-Seq
DSB-Seq
DSB-Seq maps in vivo DNA DSBs on a genome-wide scale. In this method, DNA is biotinylated by a 3’-end tailing reaction with biotin-16-dUTP. The biotinylated DNA is sonicated and captured with streptavidin-coated beads. The free DNA fragments are purified and used to prepare a sequencing library.
Pros:
- Simple protocol
Cons:
- Not yet adopted widely by the scientific community