Cancer whole-genome sequencing (WGS) provides a base-by-base view of the unique mutations present in cancer tissue. It enables discovery of novel cancer-associated variants, including single nucleotide variants (SNVs), copy number changes, and structural variants. By comparing tumor and normal DNA, WGS can also provide a comprehensive view of changes to a specific tumor sample.
Many cancer-associated variants have been discovered using cancer genome sequencing. Ongoing efforts to categorize and characterize mutations in cancer include The Cancer Genome Atlas (TCGA), the International Cancer Genome Consortium (ICGC), and Catalogue of Somatic Mutations in Cancer (COSMIC).
WGS provides base-pair resolution of an entire cancer genome and identifies SNVs, insertions and deletions (indels), and copy number variations (CNVs) in a single run. WGS provides a comprehensive view of the unique mutations in cancer tissue, including those contributed by surrounding normal tissue and tumor clonality.
Researchers use whole-genome sequencing to identify rare cancer-associated variants in the germline, in an effort to improve understanding of the genetic determinants of early-onset sarcomas.Read Article
Illumina offers several library preparation, sequencing, and data analysis options for cancer whole-genome sequencing. Streamlined library prep workflows and flexible kit configurations accommodate multiple study designs.
Approximately 90% of the world’s sequencing data are generated using Illumina sequencing by synthesis (SBS) chemistry. Push-button apps in BaseSpace Sequence Hub simplify data analysis, so you can spend less time analyzing data and more time focusing on the next breakthrough.
Click on the below to view products for each workflow step.
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.TruSeq DNA PCR-Free Library Prep Kit
Provides high genomic coverage even in challenging regions.
Enables efficient interrogation of samples with limited available DNA.
Designed to detect somatic variants from a tumor and matched normal sample pair.BaseSpace Sequence Hub
The Illumina genomics computing environment for NGS data analysis and management.
A growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and prognostic or predictive biomarkers.