AmpliSeq for Illumina Focus Panel

Targeted DNA and RNA research panel investigating 52 genes with known relevance to solid tumors.Read More...
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Panel

AmpliSeq™ Focus Panel for Illumina®

20019164

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Ampliseq™ for Illumina® Focus Panel MiSeq Kit

20024183

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Library Prep

AmpliSeq™ Library PLUS (24 Reactions) for Illumina®

20019101

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AmpliSeq™ Library PLUS (96 Reactions) for Illumina®

20019102

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AmpliSeq™ Library PLUS (384 Reactions) for Illumina®

20019103

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Index Adapters

AmpliSeq™ UD Indexes for Illumina® (24 Indexes, 24 Samples)

20019104

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AmpliSeq™ CD Indexes Set A for Illumina®

20019105

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AmpliSeq™ CD Indexes Set B for Illumina®

20019106

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AmpliSeq™ CD Indexes Set C for Illumina®

20019107

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AmpliSeq™ CD Indexes Set D for Illumina®

20019167

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AmpliSeq™ CD Indexes Set A-D for Illumina® (384 Indexes, 384 Samples)

20031676

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Accessory Products

Ampliseq™ cDNA Synthesis for Illumina®

20022654

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AmpliSeq™ for Illumina® Sample ID Panel

20019162

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AmpliSeq™ for Illumina® Direct FFPE DNA

20023378

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AmpliSeq™ Library Equalizer for Illumina®

20019171

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Product Highlights

The AmpliSeq for Illumina Focus Panel is a targeted resequencing assay for biomarker analysis of 52 genes with known relevance to solid tumors. Using the Focus Panel, researchers can analyze both DNA and RNA concurrently. Key features include:

Relevant Gene Content
  • Target biomarkers across 52 genes relevant to solid tumors
Fast, Streamlined Workflow
  • Prepare sequencing-ready libraries in a single day from as little as 1 ng of high quality or 10 ng of formalin-fixed, paraffin-embedded (FFPE) input samples
Accurate Data
  • Detect somatic mutations down to 5% frequency using local or cloud-based analysis

The Focus Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.

This ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.

Learn More About AmpliSeq for Illumina

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Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
iSeq 100 System 8 samples per run (assumes minimum coverage of 500×) 2 × 150 bp
MiniSeq System Samples per run: mid output: 16, high output: 48 (assumes minimum coverage of 500×) 2 × 150 bp
MiSeq System Samples per run (by reagent kit version): v2 micro: 8, v2: 30, v3: 48 (assumes minimum coverage of 500×) 2 × 150 bp

Product Comparison

AmpliSeq for Illumina Focus Panel AmpliSeq for Illumina Comprehensive Panel v3 AmpliSeq for Illumina Cancer Hotspot Panel v2 TruSight Tumor 15
Assay Time 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) 7 hours
Cancer Type Solid Tumor Solid Tumor Pan-Cancer, Solid Tumor Solid Tumor
Content Specifications DNA and RNA targets for 52 oncogenes DNA and RNA targets for 161 oncogenes Hotspot regions of 50 genes with known associations to cancer Amplifies 250 amplicons from 15 genes associated with solid tumors
Description Somatic analysis research on 52 genes associated with solid tumor cancers. Somatic analysis research on hotspot and full-length targets of genes associated with solid tumor cancers. Somatic analysis research into hotspot regions of 50 cancer-related genes. Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow.
Hands-On Time < 1.5 hours < 1.5 hours < 1.5 hours 3.5 hours
Input Quantity 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 1–100 ng (10 ng recommended per pool) 20 ng
Method Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing , Targeted RNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing Amplicon Sequencing , Targeted DNA Sequencing
Multiplexing 96 dual index combinations 96 dual index combinations 96 dual index combinations 1-24-plex
Nucleic Acid Type DNA, RNA DNA, RNA DNA DNA
Specialized Sample Types FFPE Tissue FFPE Tissue FFPE Tissue FFPE Tissue
Species Category Human Human Human Human

Method-Specific Workflow Example

 

Supporting Data and Figures

 

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