Comprehensive analysis of NGS amplicon data

Flexible informatics tools guide you from assay design through variant analysis

AmpliSeq for Illumina Informatics Solutions

Processing the raw sequencing data into meaningful results is straightforward with our complete informatics solutions for AmpliSeq for Illumina Panels. The analysis is designed for biologists and does not require dedicated computing infrastructure nor bioinformatics expertise.

Produce meaningful reports that include variant details and biological interpretations. Informatics solutions for both DNA and RNA amplicon panels are available.

  • With DNA amplicon panels, the secondary analysis workflow aligns reads against reference genomes and calls small variants.
  • For RNA amplicon panels, the secondary analysis workflow performs differential expression analysis and gene fusion calling.
  • Tertiary analysis adds biological context to variants, gene fusions, and differentially expressed genes with curated public and private knowledge.
User-Friendly Amplicon Data Analysis

Once the on-instrument analysis is complete, BaseSpace Sequence Hub and Local Run Manager produce variant calls and differential expression results for amplicon panels.

BaseSpace Sequence Hub

Set up and monitor instrument runs in real-time and manage and analyze data using a curated set of analysis apps:

  • Sequencing data flows directly from the instrument to the cloud
  • Converts data to a standard format
  • More economical than assembling infrastructures and tools in-house
Local Run Manager

On-instrument solution to create a sequencing run, monitor run status, analyze sequencing data, and view results:

  • Automatically performs on-instrument data analysis
  • Perform analysis procedures for different library types with optional software modules

Further downstream, Basespace Variant Interpreter and BaseSpace Correlation Engine can add biological context to your results and generate reports.

BaseSpace Variant Interpreter

Rapidly identify biologically significant variants from human genomic data:

  • Annotate data using a broad range of sources and summarize findings into structured reports
  • Analyze variants from all assay types, from whole-genome sequencing to targeted sequencing
  • Accelerate variant interpretation with over 60,000 manually-curated associations
BaseSpace Correlation Engine

Data-driven discovery of genes, experiments, drugs, and phenotypes related to genes and variants of interest:

  • Identify mechanisms of disease, drug targets, and prognostic or predictive biomarkers
  • View pathways that play a role in disease development across multiple studies and data types
  • Analyze candidate molecules for pharmacokinetic and toxicity profiles


DNA/RNA-to-Results Workflow

Streamlined Data Management and Storage with BaseSpace Sequence Hub.


Assess NGS Data Quality

On-premises data management with Local Run Manager.


Variant Analysis

Import data into BaseSpace Variant Interpreter, and generate an annotated variant report.

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