CAP-Seq
CAP-seq
CAP-seq maps the 5' end of RNAs anchored to RNAPII. In this method, RNA transcripts are treated sequentially with Terminator exonuclease, CIP, and TAP, followed by linker ligation and RT to cDNA. Deep sequencing of the cDNA provides high-resolution sequences of RNAPII transcripts.
Pros:
- Maps RNAs anchored to RNAPII
Cons:
- Multiple steps and treatments can lead to loss of material
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