TruSeq Nano DNA Library Prep Kit

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA. Read More...
Select Product(s)

TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)

FC-121-4001

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TruSeq Nano DNA LT Library Preparation Kit - Set B (12 Set B index tubes, 24 samples)

FC-121-4002

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TruSeq Nano DNA HT Library Preparation Kit (96 indexes in plate format, 96 samples)

FC-121-4003

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Product Highlights

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated library preparation
Manual preparation of high-quality libraries in less than a day

The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of traditionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexible throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

  • TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
  • TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).
  • TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications

Method-Specific Workflow Example

 

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Comparison of TruSeq DNA Library Preparation Kits
Specification TruSeq Nano DNA TruSeq DNA PCR-Free
Kit type TruSeq Nano DNA for NeoPrep TruSeq Nano DNA LT & HT TruSeq DNA PCR-Free LT & HT
Description TruSeq Nano DNA workflow performed with the NeoPrep system and reagents Based upon widely adopted TruSeq library prep, with lower input and improved data quality Superior genomic coverage with radically reduced library bias and gaps
Workflow Simplified NeoPrep workflow with lower input and less hands-on time LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
LT kits: Manual workflow
HT kits: Automation-friendly; manual protocols also available
Input quantity 25–75 ng 100–200 ng
1–2 μg
Includes PCR Yes Yes No
Assay time 7.5 hours (includes library prep through normalization) 5.5 hours ~5 hours
Hands-on time 30 minutes 4 hours ~4 hours
Target insert size 350 bp or 550 bp 350 bp or 550 bp 350 bp or 550 bp
Gel-free Yes Yes Yes
Number of samples supported Up to 16 samples per NeoPrep library card 24 (LT) or 96 (HT) 24 (LT) or 96 (HT) samples
Size-selection beads Included Included Included
Applications Whole-genome sequencing applications, including whole-genome resequencing, de novo assembly, and metagenomics studies
Sample multiplexing 24 single indexes, 16 samples per run 24 single indexes or 96 dual-index combinations
Compatible Illumina sequencers MiniSeq, MiSeq, NextSeq, HiSeq, NovaSeq, HiSeq X, HiScanSQ, and Genome Analyzer systems

Product Literature

Comparison of TruSeq Library Preparation Kits

Technical Note | PDF(< 1 MB)

TruSeq Nano DNA Library Prep Kit

Data Sheet | PDF(< 1 MB)

Available Kit Configurations

Kit types are available to accommodate a range of study designs:

  • TruSeq Nano DNA LT Library Prep Kit for low-throughput studies. The LT option is available in Set A and Set B. Each set contains 12 unique single indexes that can be pooled together for up to 24-plex.
  • TruSeq Nano DNA HT Library Prep Kit for high-throughput studies. The HT option provides dual index combinations for up to 96-plex. The index plate is single use per well. Do not undergo more than four freeze/thaw cycles. We strongly recommend processing at least 24 samples at a time.
  • Kits are also available for use with the automated NeoPrep Library Preparation System.
View TruSeq Nano DNA Library Prep Kit for NeoPrep

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