Drop-ChIP
Drop-ChIP
Droplet-based single-cell chromatin immune-precipitation sequencing (Drop-ChIP) analyzes the chromatin states of single-cells by utilizing microfluidics, unique molecular barcodes, and next-generation sequencing.
Single-cells are first isolated into droplets containing lysis buffer and MNase and then fused to a droplet carrying distinct oligonucleotides. These oligonucleotides contain the sequences for cell-specific barcodes, sequencing adaptors, and restriction sites. DNA ligase is also fused with the droplet to complete the tagging process. Then, carrier chromatins are introduced into the pooled droplets before chromatin immune-precipitation. Library preparation is carried out according to ChIP-Seq procedures before sequencing.
Pros:
- Analyzes chromatin states from single-cells in a highly parallel manner
- Unique molecular barcoding reduces the risk posed by unspecific antibodies
Cons:
- Requires a large number of sample cells