TruSeq RNA Library Prep Kit v2

These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time.Read More...
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TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)

RS-122-2001

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TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)

RS-122-2002

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Product Highlights

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex Capabilities

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal Hands-On Steps

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Automation Options

Find an up-to-date list of automation vendors with robotic systems compatible with this kit

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
2 x 75 bp
HiSeq 2500 System RNA Profiling: 60–400 samples per run (dual flow cell; based on 10 million reads per sample)
Transcriptome Analysis: 24–160 samples per run (dual flow cell; based on 25 million reads per sample)
2 x 75 bp

Product Comparison

TruSeq RNA Library Prep Kit v2 TruSeq Stranded mRNA TruSeq RNA Exome
Assay Time ~10.5 hours ~10.5 hours ~2 Days
Content Specifications Captures the coding transcriptome (without strand information) Captures the coding transcriptome with strand information Captures the coding transcriptome/RNA exome
Description A simple, cost-effective research solution for analysis of the coding transcriptome. Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information. Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement.
Hands-On Time ~4.5 hours ~4.5 hours ~11 hours
Input Quantity 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) 0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails) 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Mechanism of Action Oligo-dT beads capture polyA tails Oligo-dT beads capture polyA tails Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Method mRNA Sequencing mRNA Sequencing Exome Sequencing , mRNA Sequencing
Multiplexing Up to 24-plex per lane 1–96 Up to 24 single, 96 combinatorial (CD) dual
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible FFPE Tissue, Low-Input Samples
Species Category Bovine, Human, Mammalian, Mouse, Rat Bovine, Human, Mammalian, Mouse, Rat Human
Strand Specificity Non-Stranded Stranded Stranded

Method-Specific Workflow Example

 

Customer Stories

The Epigenetic Patterns of Exercise

Methylation analysis and RNA-Seq are helping researchers understand how endurance training makes epigenetic changes to the human genome.

Learn More

Supporting Data and Figures

TruSeq RNA Library Preparation Reagents Provide Significant Savings in Time and Effort
TruSeq RNA Library Prep Reagents number of steps

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.

 
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Related Products

TruSeq Stranded mRNA

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.


TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.


TruSeq RNA Exome

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.