TruSeq RNA Library Prep Kit v2

These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time.

~10.5 hr

Assay time

~4.5 hr

Hands-on time

0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)

Input quantity

See full details in the specifications table

Overview

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex capabilities

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal hands-on steps

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Fast, automation-friendly workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.


Specifications

Applications

Example workflow

1
Prepare

TruSeq RNA Library Prep Kit v2

2

Project recommendations

Instrument Recommended number of samples Read length
NextSeq 550 System

RNA profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome analysis: 5–16 samples per run (based on 25 million reads per sample)

2 x 75 bp


Related applications and methods

How others use this product

Compare

TruSeq RNA Library Prep Kit v2 Illumina Stranded mRNA Prep Illumina RNA Prep with Enrichment
Assay time ~10.5 hr 6.5 hr < 9 hr
Automation capability Liquid handling robots Liquid handling robots Liquid handling robots
Automation details Explore available automation methods Explore available automation methods
Content specifications Captures the coding transcriptome (without strand information) Captures the coding transcriptome with strand information Captures the coding transcriptome when used with Illumina Exome Panel
Description A simple, cost-effective research solution for analysis of the coding transcriptome. A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples
Hands-on time ~4.5 hr < 3 hr < 2 hr
Input quantity 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) 25-1000 ng standard-quality total RNA 10 ng RNA; 20 ng FFPE RNA
Instruments MiSeq System, NextSeq 550 System, NextSeq 500 System NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System MiSeq System, iSeq 100 System, NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, MiniSeq System, NovaSeq 6000 System
Mechanism of action Oligo-dT beads capture polyA tails PolyA capture, ligation-based addition of adapters and indexes Bead-linked transposome
Method mRNA sequencing mRNA sequencing mRNA sequencing, Targeted RNA sequencing, Target enrichment
Multiplexing Up to 24-plex per lane Up to 384 Unique Dual Indexes (UDIs) Up to 384 Unique Dual Indexes (UDIs)
Nucleic acid type RNA RNA RNA
Specialized sample types Not FFPE-Compatible Not FFPE-Compatible, Low-Input Samples Blood, Low-Input Samples, FFPE Tissue
Species category Mammalian, Bovine, Mouse, Human, Rat Mammalian, Bovine, Mouse, Human, Rat Human, Virus
Species details Works with high-quality RNA from any species with polyA tails Works with high-quality RNA from any species with polyA tails
Strand specificity Non-stranded Stranded Non-stranded
Technology Sequencing Sequencing Sequencing
Variant class Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants

Selection tools

Figures

TruSeq RNA Library Preparation Reagents provide significant savings in time and effort

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.

TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)

RS-122-2001

Includes reagents and 12 of 24 indexes (Indexes: 2, 4, 5, 6, 7, 12, 13, 14, 15, 16, 18, 19) for preparing 48 total RNA samples.

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TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)

RS-122-2002

Includes reagents and 12 of 24 indexes (Indexes: 1, 3, 8, 9, 10, 11, 20, 21, 22, 23, 25, 27) for preparing 48 total RNA samples.

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