TruSeq RNA Library Prep Kit v2

The TruSeq RNA Library Preparation Kits provide a simple, cost-effective solution for analysis of the coding transcriptome. Read More...
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TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)



TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)



Product Highlights

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simplified workflow with master-mixed reagents.

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples (including up to 384 RNA-Seq samples on a single HiSeq 2500 System run).

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit


Method-Specific Workflow Example


Customer Stories

The Epigenetic Patterns of Exercise

Methylation analysis and RNA-Seq are helping researchers understand how endurance training makes epigenetic changes to the human genome.

Learn More

Supporting Data and Figures

TruSeq RNA Library Preparation Reagents Provide Significant Savings in Time and Effort
TruSeq RNA Library Prep Reagents number of steps

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.


Product Literature

Manuals and Support Information

TruSeq RNA Library Prep Kit v2 Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq RNA Library Prep Kit v2 Support 

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