TruSeq RNA Library Prep Kit v2

These kits provide a simple, cost-effective solution for analysis of the coding transcriptome, with minimal hands-on time.Read More...
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TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)



TruSeq RNA Library Preparation Kit v2, Set B (48 samples, 12 indexes)



Product Highlights

Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.

Multiplex Capabilities

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.

Minimal Hands-On Steps

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.

Automation Options

Find an up-to-date list of automation vendors with robotic systems compatible with this kit


Method-Specific Workflow Example


Customer Stories

The Epigenetic Patterns of Exercise

Methylation analysis and RNA-Seq are helping researchers understand how endurance training makes epigenetic changes to the human genome.

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Supporting Data and Figures

TruSeq RNA Library Preparation Reagents Provide Significant Savings in Time and Effort
TruSeq RNA Library Prep Reagents number of steps

Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.


Product Literature

Manuals and Support Information

TruSeq RNA Library Prep Kit v2 Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq RNA Library Prep Kit v2 Support 

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Determine the best library prep kit or array for your needs based on your starting material and method of interest.

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