Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents.
Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples. The kits include adapters containing unique index sequences that are ligated to sample fragments at the beginning of the library construction process. This allows the samples to be pooled and then individually identified during downstream analysis.
Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with a user-friendly workflow.
Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners.
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 13–40 samples per run (based on 10 million reads per sample)
Transcriptome Analysis: 5–16 samples per run (based on 25 million reads per sample)
|2 x 75 bp|
|TruSeq RNA Library Prep Kit v2||Illumina Stranded mRNA Prep||Illumina RNA Prep with Enrichment|
|Assay Time||~10.5 hours||6.5 hours||< 9 hours|
|Content Specifications||Captures the coding transcriptome (without strand information)||Captures the coding transcriptome with strand information||Captures the coding transcriptome when used with Illumina Exome Panel|
|Description||A simple, cost-effective research solution for analysis of the coding transcriptome.||A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information||A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples|
|Hands-On Time||~4.5 hours||< 3 hours||< 2 hours|
|Input Quantity||0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)||25-1000 ng standard-quality total RNA||10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples|
|Mechanism of Action||Oligo-dT beads capture polyA tails||PolyA capture, ligation-based addition of adapters and indexes||Bead-linked transposome|
|Method||mRNA Sequencing||mRNA Sequencing||mRNA Sequencing, Target Enrichment, Target Enrichment, Targeted RNA Sequencing|
|Multiplexing||Up to 24-plex per lane||Up to 384 Unique Dual Indexes (UDIs)||Up to 384 Unique Dual Indexes (UDIs)|
|Specialized Sample Types||Not FFPE-Compatible||Low-Input Samples, Not FFPE-Compatible||Blood, FFPE Tissue, Low-Input Samples|
|Species Category||Bovine, Human, Mammalian, Mouse, Rat||Bovine, Human, Mammalian, Mouse, Rat||Human, Virus|
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Compared to current methods for preparing mRNA samples for sequencing, use of the TruSeq reagents significantly reduces the number of steps and hands-on time.
TruSeq RNA Sample Preparation v2 High Sample Experienced User Card and Lab Tracking Form Documentation
Illumina Experiment Manager User Guide Documentation
TruSeq RNA Sample Preparation v2 Low Sample Experienced User Card and Lab Tracking Form Documentation
TruSeq Sample Prep Best Practices Guide Documentation
Sequencing Library qPCR Quantification Guide Documentation
TruSeq RNA Sample Prep v2 Guide Documentation
TruSeq RNA Library Prep Kit v2 Documentation
User-supplied consumables and equipment excerpt from the TruSeq RNA Sample Preparation v2 Guide. Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions