hyRAD

hyRAD

Hybridization RAD (hyRAD) was developed for use on degraded samples, such as museum collections. Museums and preserved samples offer a rich source of valuable specimens, but their degraded DNA is unable to sustain the double digestion and sample fractionation required by ddRAD. To address this limitation, hyRAD uses biotinylated probes and streptavidin-covered beads to capture and enrich the fragments of interest.

The first step in the process is to generate a ddRAD library from high quality DNA, usually from an extant specimen. The fragments are size-selected and biotinylated. They can now be used as probes for hybridization capture of shotgun or ddRad libraries.

Pros:

• Can be used on degraded DNA
• Can be used on unsequenced genomes

Cons:

• Coverage not as complete as with high-quality samples

• hyRAD: Suchan T., Pitteloud C., Gerasimova N. S., Kostikova A., Schmid S., et al. (2016) Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens. PLoS One 11: e0151651

1. Suchan T., Pitteloud C., Gerasimova N. S., Kostikova A., Schmid S., et al. (2016) Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens. PLoS One 11: e0151651
2. Andrews K. R., Good J. M., Miller M. R., Luikart G. and Hohenlohe P. A. (2016) Harnessing the power of RADseq for ecological and evolutionary genomics. Nat Rev Genet
3. Holmes M. W., Hammond T. T., Wogan G. O., Walsh R. E., LaBarbera K., et al. (2016) Natural history collections as windows on evolutionary processes. Mol Ecol 25: 864-881