FastTrack Services is powered by Illumina next-generation sequencing by synthesis (SBS) HiSeq instruments, as well as TruSeq and human phasing technology, to ensure delivery of highly accurate and callable data. Additionally, our in-house software and analysis pipeline enhance data precision and research scalability. Customers benefit by receiving high-quality data from proven technologies and reduced resolution of quality scores for improved data storage capabilities and cost savings.
The FastTrack Service lab uses the TruSeq family of reagents for the entire chemistry workflow, from library preparation through DNA sequencing. The TruSeq PCR-free library prep is optimal for whole-genome sequencing.
SBS technology enables massively parallel sequencing of millions of fragments using a proprietary reversible terminator-based method that detects single bases as they are incorporated into growing DNA strands. The end result is highly accurate base-by-base sequencing across the genome including repetitive sequence regions.Learn More
HiSeq Systems offer cutting-edge scanning and imaging technology, with a high sequence output and data generation rate. They also provide a high yield of error-free reads and base calls above Q30, providing researchers with the confidence to draw sound biological conclusions.Learn More
Illumina FastTrack Services Whole-Genome Sequencing Analysis Pipeline uses the Isaac Aligner and Variant Caller to detect SNPs and small indels. Other informatics pipeline components are used to detect CNVs and SVs in whole-genome sequencing (WGS) datasets. Read more about the combined Isaac pipeline at PubMed.
In addition to identified variants, the delivered data set will include reads and reduced resolution quality score for further downstream analysis. Our quality score reduced resolution method results in a 30% reduction in the delivered BAM file size, providing customers with valuable cost and time savings in data storage and transfer, all without compromising the quality and accuracy of the results.