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Massively parallel sequencing with optimized SBS chemistry

Sequencing by Synthesis (SBS) Technology

Illumina sequencing technology, sequencing by synthesis (SBS), is a widely adopted next-generation sequencing (NGS) technology worldwide, responsible for generating more than 90% of the world's sequencing data.1 Illumina sequencing instruments and reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.

Introduction to SBS Technology

A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all 4 reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias.2

The end result is true base-by-base sequencing that enables accurate data for a broad range of applications. The method virtually eliminates errors and missed calls associated with strings of repeated nucleotides (homopolymers).

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Illumina sequencing by synthesis technology supports both single-read and paired-end libraries. SBS technology offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, and more.

The combination of short inserts and longer reads increases the ability to fully characterize any genome.

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Illumina Sequencing Introduction

This overview describes major sequencing technology advances, key methods, the basics of Illumina sequencing chemistry, and more.

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Sequencing Techniques

Explore a variety of methods enabled by SBS, from whole-genome to RNA sequencing and more. Find comprehensive workflow solutions.

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Sequencing Techniques

Find out how 2-channel SBS, a recent evolution of SBS technology, enables faster sequencing than the prior version of Illumina SBS technology, with the same high data accuracy.

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Benchtop Sequencing Machine

The NextSeq 550 System enables everyday genome, exome, transcriptome sequencing, and more.

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The Illumina semiconductor sequencing method, also known as one-channel SBS, couples sequencing by synthesis chemistry with complementary metal-oxide semiconductor (CMOS) technology.

On the iSeq 100 System, one-channel SBS on a CMOS chip allows for low instrument costs and a small instrument footprint, all while maintaining the high data accuracy of SBS.

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One-Channel SBS
MiniSeq System

Sequencing Instrument Portfolio

Illumina offers innovative sequencing systems that deliver exceptional data quality and accuracy, at the scale you need.

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NextSeq500 v2 Kits
Improve Your Sequencing Results

NextSeq 500/550 v2.5 Kits offer increased stability and robustness over the v2 kit version.

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Patterned Flow Cell Technology
Maximum Throughput

See how patterned flow cell technology dramatically increases sequencing data throughput.

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  1. Data calculations on file. Illumina, Inc. 2015.
  2. Bentley DR, Balasubramanian S, Swerdlow HP, et al. Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry. Nature. 2008; 456 (7218): 53–59.