System specifications for NextSeq 550Dx

The reliability and consistency of a clinical instrument

NextSeq 550Dx Specifications

Read Length 2 × 150 bp
Output ≥ 90 Gb
Total Time* ≤ 35 hours
Throughput 8–96 samples/run depending on assay

* Includes cluster generation, 2 × 150 bp dual-indexed sequencing, reagent purge, and post-run wash.

High-Output ≥ 600 M paired-end reads
Bases with ≥ Q30 > 75%
Total Overall Accuracy – Somatic Variant Caller ≥ 99.98%
Total Overall Accuracy – Germline Variant Caller ≥ 99.99%

† Using a representative assay designed to query a variety of genes covering approximately 13,000 bases across 23 different chromosomes, and containing wide GC content (18% to 87%), indels (up to 25 base pairs), and homopolymers (7 to 13 nucleotides).

Power Requirements 100–240 Volts AC @ 50/60 Hz, 600w max
RFID Radio Frequency 13.56 MHz
RFID Power 200 mW
LED 520 nm, 650 nm; Laser diode: 780 nm, Class IIIb
Laser Class 1 laser product embedded with a Class 3B diode
Temperature Transportation and Storage: -10°C to 50°C.
Operating Conditions: Maintain a lab temperature of 19°C to 25°C (22°C±3°C).
RFID Radio Frequency 13.56 MHz
W×D×H 54 cm × 69 cm × 58 cm
Weight (84.4 kg) 186 lbs
Crated Weight (163 kg) 360 lbs
Base Unit Dual Intel Xeon E5-2648L v3
CPU 1.8 GHz
Memory 128 GB
Hard Drive 2 × 2 TB
Operating System Windows 10

Specifications shown here are for IVD use. View research mode only specs, applicable only when running the NextSeq 550Dx instrument in in Research mode.

NextSeq 550Dx System Specification Sheet

NextSeq 550Dx System Specification Sheet

Learn more about the workflow and flexibility of the NextSeq 550Dx; perform both IVD testing and clinical research on the same instrument.

View Specification Sheet
Sequencing by Synthesis Technology

The NextSeq 550Dx System harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.

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2-Channel SBS Technology

The NextSeq 550Dx System leverages 2-channel SBS chemistry to offer efficient sequencing and data generation. 2-channel SBS reduces cycle and data processing times, while delivering the same high quality and accuracy that sets Illumina systems apart.

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Intended Use for the NextSeq 550Dx

United States:
The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx instrument is to be used with registered and listed, cleared or approved, IVD reagents and analytical software.

European Union:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved IVD reagents and analytical software.