| Read length | NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles) |
|---|---|
| 2 × 150 bp | 120 Gb |
NextSeq 550Dx Instrument specifications
The reliability and consistency of a clinical instrument
Delivering high-quality, reliable data for diagnostic testing using proven SBS chemistry
Output per flow cell
NextSeq 550Dx Instrument
Reads passing filter per flow cell
NextSeq 550Dx Instrument
| NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles) | |
|---|---|
| Paired-end reads | ≥ 600M |
Quality scoresa
NextSeq 550Dx Instrument
| Read length | NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles) |
|---|---|
| 2 × 150 bp | > 75% bases higher than Q30 |
a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
Run timea
NextSeq 550Dx Instrument
| Read length | NextSeq 550Dx High-Output Reagent Kit v2.5 (300 cycles) |
|---|---|
| 2 × 150 bp | ≤ 35 hr |
Total overall accuracya
NextSeq 550Dx Instrument
| Somatic variant caller | ≥ 99.98% |
| Germline variant caller | ≥ 99.99% |
a. Using a representative assay designed to query a variety of genes covering approximately 13K bases across 23 different chromosomes and containing wide GC content (18%–87%), indels (up to 25 base pairs), and homopolymers (7–13 nucleotides).
Instrument specifications
-
Output range
16.25 - 120 Gb
-
Reads per run
130 - 400 million
-
Max read length
2 × 150 bp
-
Run time
12 - 30 hr
-
Light emitting diode (LED)
Wavelengths
(laser diode 780 nm, Class IIIb)Green 510–525 nm, red 645–655 nm
-
Dimensions
W × D × H54 cm × 69 cm × 58 cm
(21.3 in × 27.2 in × 22.8 in) -
Weight
84.4 kg (186 lb)
-
Crated weight
163 kg (360 lb)
Specifications shown here are for IVD use. View research only specifications, applicable when running the NextSeq 550Dx Instrument in Research Mode.
Key technologies
Sequencing by synthesis chemistry
The NextSeq 550Dx Instrument harnesses proven Illumina sequencing by synthesis (SBS) technology to deliver highly accurate data and robust performance for multiple applications. SBS uses a reversible-terminator method with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.
2-channel sequencing by synthesis
The NextSeq 550Dx Instrument leverages 2-channel sequencing by synthesis (SBS) for faster data generation than the original Illumina 4-channel SBS technology, with the same high data accuracy.
Intended Use for the NextSeq 550Dx Instrument
United States:
The NextSeq 550Dx Instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue when used with in vitro (IVD) diagnostic assays. The NextSeq 550Dx Instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx Instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.
European Union:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic assays. The NextSeq 550Dx instrument is to be used with specific registered, certified, or approved in vitro diagnostic reagents and analytical software.