All Illumina sequencing systems use our proven sequencing by synthesis (SBS) technology. SBS is the most widely adopted next-generation sequencing (NGS) technology, producing more than 90% of global sequencing data.* SBS delivers highly accurate base-by-base sequencing and robust performance across the genome.
Several Illumina instruments use a recent evolution of SBS technology known as 2-channel SBS. This method enables faster data generation with the same high data accuracy as 4-channel SBS.
The 2-channel SBS method requires only 2 images per cycle, instead of 4 (Figure 1). This accelerates sequencing and data processing times, while delivering the same quality and accuracy that sets Illumina systems apart. These advances make 2-channel SBS an affordable solution, enabling NGS to become an everyday tool in laboratories worldwide.
Rather than a separate dye for each base, 2-channel SBS uses a mix of dyes. Images are taken of each DNA cluster using red and green wavelength filter bands (Figure 2).
Clusters seen in red or green images are interpreted as C and T bases, respectively. Clusters observed in both red and green images are flagged as A bases (appearing as yellow clusters), while unlabeled clusters are identified as G bases.
The MiniSeq System leverages 2-channel SBS to offer a small, affordable benchtop sequencer. It supports a wide range of applications, from targeted DNA and RNA sequencing to tumor profiling studies, small RNA discovery, and more.
The NextSeq Series delivers speed and simplicity for everyday genomics, leveraging 2-channel SBS to offer efficient sequencing and data generation. These benchtop sequencers support whole-transcriptome, exome, and targeted resequencing studies.
The NovaSeq 6000 System offers scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.
One-channel SBS couples sequencing by synthesis chemistry with complementary metal-oxide semiconductor (CMOS) technology. While it uses the same base-calling architecture as two-channel SBS, the one-channel method supports lower instrument costs and the convenience of a small instrument footprint, all while maintaining high accuracy. Our smallest sequencer, the iSeq 100 System, uses one-channel SBS.
* Data calculations on file. Illumina, Inc. 2015.