NovaSeq 6000Dx Instrument Specifications

IVD-compliant high-throughput sequencing for the clinical lab

Take a closer look at the output, run time, read length, sample throughput, and other NovaSeq 6000Dx specifications

Instrument Performance Parameters—IVD Mode

Output per Flow Cell

NovaSeq 6000Dx Instrument Performance Parameters(•,†)
Flow Cell Type Read Length Output Reads Per Run(‡) Run Time Data Quality (¶)
S2 2 x 150 bp ≥ 1 Tb ≥ 6.67 B ≤ 40 hours ≥ 85% Q30
S4 2 x 150 bp ≥ 3 Tb ≥ 20 B ≤ 45 hours ≥ 85% Q30

• Libraries generated with Illumina DNA Prep with Enrichment Dx.
† For performance parameters in RUO mode, see NovaSeq 6000 System specifications.
‡ Paired-end reads per flow cell.
¶ A quality score of Q30 corresponds to an error rate of 1 in 1000 bases called.

Variant Calling Performance

NovaSeq 6000Dx Instrument
  Germline variant calling Somatic variant calling
SNV Positive Percent Agreement (%) ≥ 99.8% ≥ 99.8%
Insertion PPA (%) ≥ 97.9% 100%
Deletion PPA (%) ≥ 96.9% 100%
Negative Percent Agreement (%) > 99.9% > 99.9%
Overall Percent Agreement (%) > 99.9% > 99.9%

*Variant calling performance results obtained using a representative assay designed to query a variety of genes covering approximately 2 million bases across 23 different chromosomes, and containing wide GC content (20% to 86%), insertions (up to 18 base pairs), and deletions (up to 21 base pairs).

NovaSeq 6000Dx Instrument Specifications

Instrument configuration
  • Computer and touch screen display
  • Installation setup and accessories
  • Data collection and analysis software
Instrument control computer
  • Base Unit: Axiomtek MANO525 with i7-8700T CPU
  • Memory: 2 x 8 GB DDR4 SODIMM
  • Hard Drive: None
  • Solid-State Drive: 256 GB 2242 M.2
  • Operating System: Windows 10
  • Note: Computer configurations will be upgraded regularly; contact your local account manager for current configuration
Operating environment
  • Temperature: 19°C-25°C (22°C ±3°C), <2°C change per hour
  • Humidity: 20%-80% relative humidity, non-condensing
  • Altitude: Below 2000 meters (6500 feet)
  • Ventilation: Maximum of 8530 BTU/h and average 6000 BTU/h
  • For Indoor Use Only
  • Class 1 laser product embedded with class IV lasers: 532 nm, 660 nm, 780 nm, 790 nm
  • W × D × H: 80.0 cm (31.5 in) × 94.5 cm (37.2 in) × 165.6 cm (65.2 in) with monitor
  • Weight: 481 kg (1059 lb), includes 3.5 kg (7.8 lb) for leak tray and 0.9 kg (2 lb) for keyboard and mouse, Crated Weight: 628 kg (1385 lb)
Power requirements
  • 200–240 VAC 50/60Hz, 16A, single phase, 2500 W
  • Illumina provides a region-specific uninterruptible power supply
Radio frequency identifier (RFID)
  • Frequency: 13.56 MHz
  • Power: supply 3.3 volts DC ± 5%, current 120 mA, RF output power 200 mW
Network connection
  • Dedicated 1 Gb connection between the instrument and data management system. Connect directly or through network
Bandwidth for network connection
  • 200 Mb/s/instrument for internal network uploads
  • 200 Mb/s/instrument for BaseSpace Sequence Hub uploads
  • 5 Mb/s/instrument for Instrument Operational Data uploads
View In-Depth Spec Sheet (PDF)
novaseq 6000 system drawing

Specifications shown here are for IVD use. View Research Mode only specs, applicable only when running the NovaSeq 6000Dx instrument in Research Mode.

Key Technology

Accurate and Efficient Data Analysis Technology

Each NovaSeq 6000Dx instrument comes with a paired dedicated DRAGEN Server and included license for accelerated secondary analysis. With significant advancements in hardware acceleration, lossless compression, and technology innovation, DRAGEN is setting new standards for accuracy and increased genome coverage to deliver actionable insights faster.

Run Management Technology

Illumina Run Manager for NovaSeq 6000Dx is fully integrated with the sequencing system. Accessed either on-instrument or remotely, this intuitive interface enables control of user and instrument management, application configuration, run setup, and secondary analysis modules on the DRAGEN Server. Plan and start your sequencing runs in IVD and RUO Modes, track libraries with audit trails, and monitor run progress.

Sequencing by Synthesis Technology

NovaSeq 6000Dx leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

Learn More

Patterned Flow Cell Technology

NovaSeq 6000Dx incorporates patterned flow cell technology to generate a high level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.

Learn More

Interested in learning more about the power of NovaSeq 6000Dx?

Talk to an Illumina representative today.

Speak to a Specialist