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| NovaSeq 6000 System | ||||
|---|---|---|---|---|
| Flow Cell Type | SP*† | S1 | S2 | S4 |
| 2 × 50 bp | 65–80 Gb | 134–167 Gb | 333–417 Gb | N/A‡ |
| 2 × 100 bp | N/A‡ | 266–333 Gb | 667–833 Gb | 1600–2000 Gb† |
| 2 × 150 bp | 200–250 Gb | 400–500 Gb | 1000–1250 Gb | 2400–3000 Gb |
Specifications based on Illumina PhiX control library at supported cluster densities.
* The NovaSeq 6000 SP Reagent Kits have not been released; therefore, performance metrics are subject to change.
† The NovaSeq 6000 SP Reagent Kits and NovaSeq 6000 S4 Reagent Kits (200 cycles) will be coming soon.
‡ N/A: not applicable
| NovaSeq 6000 System | ||||
|---|---|---|---|---|
| Flow Cell Type | SP* | S1 | S2 | S4 |
| Single-end Reads | 650–800 M | 1.3–1.6 B | 3.3 B–4.1 B | 8-10 B |
| Paired-end Reads | 1.3–1.6 B | 2.6–3.2 B | 6.6–8.2 B | 16–20 B |
* The NovaSeq 6000 SP Reagent Kits have not been released; therefore, performance metrics are subject to change.
| NovaSeq 6000 System | ||||
|---|---|---|---|---|
| Flow Cell Type | SPठ| S1 | S2 | S4 |
| Quality Scores | ||||
| 2 × 50 bp | ≥ 85% | |||
| 2 × 100 bp | ≥ 80% | |||
| 2 × 150 bp | ≥ 75% | |||
| Run Time | ||||
| 2 × 50 bp | ~13 hr | ~13 hr | ~16 hr | N/A |
| 2 × 100 bp | N/A | ~19 hr | ~25 hr | ~36 hr§ |
| 2 × 150 bp | ~25 hr | ~25 hr | ~36 hr | ~45 hr |
* A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run. Quality scores are based on NovaSeq Reagent Kits run on the NovaSeq 6000 System using an Illumina PhiX control library. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
† Run time includes cluster generation, sequencing, and base calling. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time.
‡ The NovaSeq 6000 SP Reagent Kits have not been released; therefore, performance metrics are subject to change.
§ The NovaSeq 6000 SP Reagent Kits and NovaSeq 6000 S4 Reagent Kits (200 cycles) will be coming soon.
| NovaSeq 6000 System | ||||
|---|---|---|---|---|
| Flow Cell Type | SP†‡ | S1 | S2 | S4‡ |
| Human Genomes per Run | ~4 | ~8 | ~16 | ~48 |
| Exomes per Run | ~40 | ~80 | ~200 | ~500 |
| Transcriptomes per Run | ~32 | ~64 | ~164 | ~400 |
* All sample throughputs are estimates and are based on dual flow cell runs. Human Genomes assumes > 120 Gb of data per sample to achieve 30× genome coverage. Exome assumes ~8Gb/100×. Transcriptomes assume ≥ 50M reads.
† The NovaSeq 6000 SP Reagent Kits have not been released; therefore, performance metrics are subject to change.
‡ The NovaSeq 6000 SP Reagent Kits and NovaSeq 6000 S4 Reagent Kits (200 cycles) will be coming soon.
The NovaSeq 6000 Sequencing System offers scalable throughput and flexibility for virtually any sequencing method, genome, and scale. Complete projects faster and more economically than ever before—and redefine the limits of your research.
View Specification SheetThe NovaSeq 6000 System leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
See How SBS WorksThe NovaSeq 6000 System incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.
See Patterned Flow Cells in Action