|NovaSeq 6000 System|
|Flow Cell Type||SP †||S1||S2||S4|
|Human Genomes per Run||~4||~8||~20||~48|
|Exomes per Run||~40||~80||~200||~500|
|Transcriptomes per Run||~32||~64||~164||~400|
* All sample throughputs are estimates and are based on dual flow cell runs. Human Genomes assumes > 120
Gb of data per sample to achieve 30× genome coverage. Exome assumes ~8Gb/100×. Transcriptomes assume
≥ 50M reads. Throughput may vary based on library preparation kit used.
† The NovaSeq 6000 SP Reagent Kits have not been released; therefore, performance metrics are subject to change.
The NovaSeq 6000 Sequencing System offers scalable throughput and flexibility for virtually any sequencing method, genome, and scale. Complete projects faster and more economically than ever before—and redefine the limits of your research.View Specification Sheet
The NovaSeq 6000 System leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.See How SBS Works
The NovaSeq 6000 System incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.See Patterned Flow Cells in Action