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NovaSeq System Specifications

Sequencing Output per Flow Cell

	NovaSeq 6000 System
Flow Cell Type	S1	S2	S4
2 × 50 bp	134–167 Gb	333–417 Gb	N/A*
2 × 100 bp	266–333 Gb	667–833 Gb	N/A*
2 × 150 bp	400–500 Gb	1000–1250 Gb	2400–3000 Gb

Specifications based on Illumina PhiX control library at supported cluster densities.
* N/A: not applicable

Reads Passing Filter Per Flow Cell

	NovaSeq 6000 System
Flow Cell Type	S1	S2	S4
Single-end Reads	1.3–1.6 B	3.3 B–4.1 B	8-10 B
Paired-end Reads	2.6–3.2 B	6.6–8.2 B	16–20 B

Quality Scores^* and Run Time^†

	NovaSeq 6000 System
Flow Cell Type	S1	S2	S4
Quality Scores
2 × 50 bp	≥85%
2 × 100 bp	≥80%
2 × 150 bp	≥75%
Run Time
2 × 50 bp	~13 hr	~16 hr	N/A
2 × 100 bp	~19 hr	~25 hr	N/A
2 × 150 bp	~25 hr	~36 hr	~45 hr

* A quality score (Q-score) is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run. Quality scores are based on NovaSeq Reagent Kits run on the NovaSeq 6000 System using an Illumina PhiX control library. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
† Run time includes cluster generation, sequencing, and base calling. Run times are based on running 2 flow cells of the same type; starting two different flow cells will impact run time.

Estimated Sample Throughput for Key Applications^*

	NovaSeq 6000 System
Flow Cell Type	S1	S2	S4
Human Genomes per Run	~8	~16	~48
Exomes per Run	~80	~200
Transcriptomes per Run	~64	~164

* All sample throughputs are estimates and are based on dual flow cell runs. Human Genomes assumes > 120 Gb of data per sample to achieve 30× genome coverage. Exome assumes ~8Gb/100×. Transcriptomes assume ≥ 50M reads.

Sequencing by Synthesis Technology

The NovaSeq 6000 System leverages proven Illumina sequencing by synthesis (SBS) technology to deliver accurate data and robust performance. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides. This method detects single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.

See How SBS Works

Patterned Flow Cell Technology

The NovaSeq 6000 System incorporates patterned flow cell technology to generate an unprecedented level of throughput for a broad range of sequencing applications. Patterned flow cells contain billions of nanowells at fixed locations, a design that provides even spacing of sequencing clusters. This delivers significant increases in sequencing reads and total output of the system.

See Patterned Flow Cells in Action