Genetic markers linked to specific value traits can be used to screen large numbers of progeny to identify those with desired characteristics. Trait screening is ideal for multigenic traits that are difficult to manage using conventional breeding or propagation techniques, and even more difficult to identify phenotypically. Iterative screening of populations enables easier segregation of progeny possessing the desired traits for use as breeding stock.
The goal of backcrossing is to move a single trait of interest, such as drought-tolerance, high-productivity, or disease resistance, from a donor parent to progeny. Marker-assisted backcrossing enables researchers to monitor the transmission of the trait gene via a genetically-linked marker that can be easily screened, significantly accelerating backcrossing programs and reducing the time to release of commercially viable plant lines or breeding stock.
Animal identity verification and parentage are essential tools in maintaining the biosecurity of the world’s animal populations. Illumina offers fast, affordable, reliable high-throughput genotyping platforms that can be used for virtually any animal species.
The recent emergence of novel infectious diseases in animal populations has forced researchers to look beyond conventional diagnostic tests for ways to identify these new pathogens. The speed and affordability of next generation sequencing (NGS) has fueled the field of metagenomics. The MiSeq Sequencing System provides an effective platform for screening samples to detect infectious agents and is fast becoming a useful tool in veterinary applications.
Genetic markers can be used to identify animals and understand the relationship of offspring to parents. Since a single marker may not yield definitive results, multiple markers are used to increase the probability of identifying the true parent. In linebreeding situations where multiple generations of males or females are present in the herd, the marker results are combined with the breeder’s knowledge of possible sires or dams to determine parentage.
The TruSeq Bovine Parentage Sequencing Panel enables parentage testing of multiple beef and dairy cattle breeds, with added genotyping of relevant traits in a single assay.
Molecular characterization of genetically modified organisms (GMO) is necessary for event selection and, in many countries, regulatory approval. The simplicity and consistency of NGS makes this method a superior alternative to the existing process of Southern blotting and sequencing of PCR products.
Deep sequencing of the whole genome allows determination of the number of insert sites, copy numbers at each site, sequence of the insert and the native site, and analysis of generational stability.