Traditionally, cell biology research seeks to understand the contribution/function of a single gene, gene family, or signaling pathway to a complex disease. Researchers use signal-based methods such as microscopy, flow cytometry, protein blotting, and others. Given the multifactorial nature of complex diseases, this approach can be time-consuming and does not always result in conclusive findings.
Array and next-generation sequencing (NGS) technologies from Illumina can broaden cellular research beyond the conventional methods of protein interactions and single-gene functional studies. Illumina technology enables analysis across the genome, transcriptome, and epigenome. NGS results can inform experimental design and subsequent studies, saving time and allowing researchers to publish sooner.
SureCell Whole Transcriptome Analysis 3' Library Prep Kit: This kit supports transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.
NextSeq 550 System: This system combines sequencing and array scanning on a single platform.
BaseSpace Sequence Hub Single-Cell RNA-Seq App: Designed for use with the SureCell WTA 3' Library Prep Kit, this app performs cell and gene counting, filtering, and reporting.