Focused investigation of key genes

Sequencing targeted genomic regions of interest reduces sequencing costs, time, and data analysis

Targeted Resequencing

With targeted resequencing, a subset of genes or regions of the genome are isolated and sequenced. Targeted approaches using next-generation sequencing (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA.

Get Started with Targeted NGS
Get Started with Targeted NGS

This video walks you through the entire targeted next-generation sequencing (NGS) workflow—from creating your design, to prepping your libraries, sequencing, and then analyzing your data.

  • Focuses on regions of interest, generating a smaller, more manageable data set
  • Reduces sequencing costs and data analysis burdens
  • Reduces turnaround time compared to broader approaches
  • Enables deep sequencing at high coverage levels for rare variant identification

Compared to broader approaches, such as whole-genome sequencing, targeted sequencing is a more cost-effective method for investigating areas of interest. Targeted resequencing enables researchers to focus on regions that are most likely to be involved in the phenotype under study, conserving resources and generating a smaller, more manageable data set. Targeted approaches can also deliver much higher coverage levels, allowing identification of variants that are rare and more expensive with whole-genome or Sanger sequencing.

Benefits of Targeted Resequencing

Targeted resequencing is simpler and more accessible than ever before. Find out how to access the focused power of targeted NGS.

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Using qPCR or Sanger sequencing today?

Find out how Illumina targeted sequencing can help you discover a lot more.

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How can I use NGS to analyze specific genes or DNA regions?

Explore common targeted NGS methods, from exome to amplicon sequencing and more.

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Compare amplicon and enrichment sequencing 

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How can I apply targeted NGS?

Discover, validate, or screen genetic variants with targeted sequencing for various research areas, from cancer to microbiology and more.

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Sequencing Method Explorer

Use this interactive tool to explore experimental NGS library preparation methods compiled from the scientific literature.

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Sequencing Method Explorer

Somatic mutations in cerebral cortical malformations.

N Engl J Med 371 733-43 2014

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Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform.

Sci Rep 5 9687 2015

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Identification of minority resistance mutations in the HIV-1 integrase coding region using next generation sequencing.

J Clin Virol 73 95-100 2015

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Advancing Clinical Cancer Research with NGS
Advancing Clinical Cancer Research with NGS

Targeted NGS enables researchers to cost-effectively assess multiple genes in a single test.

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Gene Panel Finder
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Identify sequencing panels that target your genes of interest.

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Basics of NGS for FFPE Tumor Specimens
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Learn how to get started using targeted NGS assays in your clinical research laboratory.

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Sequencing Platform Comparison Tool
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Compare the speed and throughput of Illumina sequencing systems to find the ideal platform for your lab.

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Illumina NGS Application Guide
Illumina NGS Methods Guide

All the information you need, from library preparation to sequencer selection to analysis. Select the best tools for your lab.

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Sequencing Troubleshooting Tips
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View Sequencing Coverage Tips
View Sequencing Coverage Tips

Learn how to estimate and achieve the necessary sequencing coverage for your experiment.

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High-Throughput Detection of Somatic Mosaicism
Detection of Somatic Mosaicism

Targeted resequencing is enabling a high-throughput approach to identify rare mosaic mutations in brain malformations.
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