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Ultra–high–resolution HLA typing

HLA Sequencing

Sequencing the HLA region can provide critical insight into immune disorders. Achieving high-resolution human leukocyte antigen (HLA) typing results with conventional methods requires multiple assays, systems, and analysis programs.

HLA typing by next-generation sequencing (NGS) generates unambiguous, phase-resolved HLA typing results using a single assay, system, and analysis program.

A New Paradigm in HLA Sequencing
A New Paradigm in HLA Sequencing

Achieve high accuracy, efficiency, and certainty in HLA typing—all in a single assay.

The HLA region is the most densely polymorphic region of the genome. Sequencing HLA genes used to be notoriously difficult due to high levels of sequence homology between HLA genes and pseudogenes, dense variability, and poor characterization of the thousands of HLA alleles in the population.

NGS with Illumina sequencing by synthesis (SBS) chemistry overcomes these challenges, enabling simple, high-quality analysis of the key HLA genes. Highly accurate and robust Illumina sequencing by synthesis (SBS) chemistry is the most successful and widely adopted NGS technology worldwide.*

Targeted Sequencing

In principle, NGS is similar to Sanger (capillary electrophoresis-based) sequencing. The bases of a DNA fragment are identified sequentially from signals emitted as each fragment is resynthesized from a DNA template strand. NGS scales up this process; millions of reactions occur in a massively parallel fashion. This advance enables rapid sequencing of large stretches of DNA such as the HLA region.

Learn More About NGS Technology

Illumina NGS technology supports paired-end sequencing, a unique feature that is crucial for successful, unambiguous HLA typing. Sequencing the ends of the library DNA fragments generates high-quality base calls. The physical link between the 2 reads (originating from the same clonally amplified library DNA fragment) allows association of variants found in each read pair. The distance between the paired reads varies as a result of the random library fragment generation process, allowing the direct resolution of the phase of 2 variants.

Learn More About Paired-End Sequencing

Featured HLA Sequencing Research

 
Tumor-Specific HLA Ligands
Precision Immunotherapies Using Tumor-Specific HLA Ligands

RNA-Seq and HLA typing are increasing the power and efficiency of a target discovery platform.

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Targeted DNA Analysis
HLA Genetics & Autoimmune Disease

Researchers query the genome with NGS and perform HLA genotyping to identify novel risk loci for immune-mediated disease.

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DNA sequencing
Exploring HLA Diversity in Brazil

Drawing upon the genetic diversity of Brazilians, researchers develop an HLA variation database.

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Multiplexing enables large numbers of loci to be sequenced simultaneously during a single experiment. Individual “barcode” sequences are added to each locus so they can be differentiated during HLA sequencing data analysis.

Learn More About Multiplex Sequencing

The TruSight HLA v2 Sequencing Panel enables phase-resolved, sample-to-report HLA typing for 11 loci in a single assay, with a single workflow, on a single instrument, for dozens of samples simultaneously. Gone are the days of resolving ambiguities for every sample at every locus; TruSight HLA delivers an ultra-high–resolution typing result the first time.

Gain Insight into Immunological Diseases

NGS and microarray solutions enable researchers to investigate potential autoimmune disease mechanisms, the immune repertoire, tumor immunogenicity, and the functional consequences of immune-related genetic variation.

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immuno-oncology research
  • MiniSeq System: With a low purchase price and cost-efficiency even for low sample numbers, MiniSeq enables a broad range of targeted DNA and RNA applications.
  • MiSeq System: Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more.
  • NextSeq 550 System: A high-throughput benchtop sequencer that enables whole-genome, transcriptome, and targeted resequencing plus the ability to scan select microarrays.

See All Benchtop Sequencers

HLA Sample to Report
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Introducing TruSight HLA
Evolution of HLA Typing

The TruSight HLA sequencing solution offers streamlined, economical, and high-quality ultra-high–resolution HLA typing.

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Illumina Sequencing by Synthesis
Illumina Sequencing by Synthesis

Illumina SBS chemistry has revolutionized the study of genomics. See SBS chemistry in action.

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TruSight HLA v2 Whitepaper
White Paper: HLA Sequencing

Results of repeated sequencing of 72 International Histocompatibility Working Group (IHWG) reference samples.

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*Data calculations on file. Illumina, Inc., 2015