Focus the power of NGS
Powerful and compact, the MiSeq System is ideal for rapid, cost-effective next-generation sequencing for virtually any lab
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We are making the MiSeq Sequencing System obsolete. It will be available for order until September 30, 2025. We will continue to provide full system support through December 31, 2029. For more information, refer to PON2025-1657. The MiSeq i100 Series is the recommended alternative.
MiSeq System is a benchtop sequencing system capable of performing a broad range of sequencing applications. Optimized sample preparation kits, push-button operation, and automated data analysis create a fast and easy end-to-end sequencing workflow for any lab.
Simplicity at the touch of a button. Easy-to-follow software, onboard data analysis, and fast and easy data sharing streamline genetic analysis on the MiSeq System.
With adjustable read lengths, multiple flow cell options, and choice of single or paired-end reads, the MiSeq System provides the flexibility to match data output to experimental needs.
Go from DNA to data in just over eight hours. The MiSeq System incorporates cluster generation, paired-end fluidics, sequencing by synthesis chemistry, and data analysis in a compact, all-in-one platform.
System specifications
Manage workflow seamlessly with Clarity LIMS or an existing LIMS system.
Prepare sequence-ready libraries with optimized library prep kits for targeted applications.
Generate 2 × 300 bp paired-end reads in a single run.
Complete analysis reports in a few hours with onboard data analysis tools.
Monitor and share data in real time with colleagues during a MiSeq System sequencing run.
With automated paired-end reads, an output range of up to 15 Gb per run, and optimized library prep kits, the MiSeq System can accommodate a variety of applications, including targeted gene sequencing, small genome sequencing, amplicon sequencing, 16S metagenomics analysis, and more.
A targeted deep sequencing assay identifies multidrug-resistant tuberculosis strains responsible for silent outbreaks.
See how 16S rRNA sequencing on the MiSeq System enables studies like The American Gut Project.
MiSeq System |
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| Run time | 4–55 hr | ~4-15.5 hr | 4–24 hr |
| Maximum output per run | 15 Gb | 30 Gba | 7.5 Gb |
| Maximum single reads per run | 25Mb | 100M | 25Mc |
| Maximum read length | 2 × 300 bp | 2 × 300 bp | 2 × 150 bp |
| Data analysis offeringsd | MiSeq Reporter Software, BaseSpace Sequence Hub, Illumina Connected Analytics, on-premises DRAGEN server | Popular DRAGEN applications available on instrument, broader menu on cloud (BaseSpace Sequence Hub and Illumina Connected Analytics), on-premises DRAGEN server. | BaseSpace Sequence Hub, Illumina Connected Analytics, on-premises DRAGEN server |
A fast, integrated workflow for preparing libraries for use in a wide range of sequencing applications.
Optimized reagent kit that provides increased cluster density and read length, improving sequencing quality scores compared to earlier versions.
Reagent kits that provide increased cluster density and read length for the MiSeq System, improving sequencing quality scores compared to v1 kits.
Offers a 90-min workflow to prepare DNA libraries for small genomes, PCR amplicons, plasmids, and cDNA sequencing, with a low DNA input requirement.
Get assistance with deciding if this powerful and compact sequencing system is right for your lab. Contact us today for more information on the MiSeq System.
Your email address is never shared with third parties.
