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MiSeq System

Broad Applications Base

The MiSeq System facilitates your research with a wide range of sequencing applications. It is capable of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.

Explore Applications

Next: Integrated, Optimized Library Prep

Integrated, Optimized Library Prep

Library prep kits prepare input RNA or DNA for a diverse range of applications such as whole-genome and targeted region resequencing, transcriptome analysis, and expression profiling. Each library prep method is optimized to fit each unique application, and most are available in a variety of throughput and multiplexing configurations.

Next: Simple NGS Workflow

Simple NGS Workflow

The MiSeq System can go from DNA to data in just over 8 hours. A compact, all-in-one platform incorporates cluster generation, paired-end fluidics, sequencing by synthesis chemistry, and data analysis. An intuitive touch screen and plug-and-play reagents with RFID tracking add convenience. The MiSeq System eliminates the need for auxiliary hardware and computing resources, saving valuable lab bench space. Seamless data upload to BaseSpace Sequence Hub enables analysis, collaboration, and security.

Next: User-Friendly, Intuitive Software

User-Friendly, Intuitive Software

System software guides you through sample sheet creation and setup prior to a run, then walks you through flow cell and reagent loading steps. Control software overviews quality statistics you can monitor as the run progresses. Once primary analysis completes, reporting software launches and automatically analyzes the data quality and coverage information of each sample.

Start Over: Broad Applications Base
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Workflow

 
Prep

Library prep kits are optimized for targeted applications.

 
Sequence

MiSeq System can generate 2 × 300 bp paired-end reads in a single run.

 

 
Analyze

Selected analysis initiates automatically, completes in a few hours, then generates a report.

 
Share

Monitor and share data in real time with colleagues during a MiSeq run.

See MiSeq Application-Specific Workflows

System Specifications

540 Mb–15 Gb
output range

1-25 million
reads per run

2 × 300 bp
max read length

View All MiSeq Specifications

Customer Stories

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Of Humans and Microbes: a Long Lasting Relationship

Researchers analyzed fecal samples from humans, chimpanzees, bonobos, and gorillas to demonstrate that hominids and certain gut colonizing bacteria co-evolved.

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Seeking the source of bacterial drug resistance

A targeted deep sequencing assay identifies multidrug-resistant tuberculosis strains responsible for silent outbreaks

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Streamlined workflow management enables sequencing at scale

Implementation of BaseSpace Clarity LIMS Software enables Rapid Novor to future-proof their growing laboratory operations

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