DRAGEN v4.0 Updates | New features include
New DRAGEN v4 Server:
DRAGEN Azure Lvl 4 1 year License
99.83% accuracy score with the Precision FDA Truth Challenge V2 benchmarking data.3
Analyze whole genomes, exomes, methylomes, and transcriptomes with a single platform.
Process a 34x genome in ~ 30 minutes, with all supported callers2. Reduce FASTQ file sizes up to 5× with DRAGEN ORA Compression
The DRAGEN team has introduced powerful machine learning (ML) and improved graph genome mapping. These new advancements propel DRAGEN to lead accuracy across all read technologies in all benchmark regions and the MHC region. In this blog, we show the results collected in the PrecisionFDA Truth Challenge V2, compare the latest DRAGEN accuracy against the challenge submissions across all read technologies, and describe the methods used to reach high accuracy levels.Read Blog
The DRAGEN Platform enables labs of all sizes and disciplines to do more with their genomic data. The DRAGEN Platform uses highly reconfigurable field-programmable gate array technology (FPGA) to provide hardware-accelerated implementations of genomic analysis algorithms, such as BCL conversion, mapping, alignment, sorting, duplicate marking, and haplotype variant calling. DRAGEN ORA lossless genomic compression technology enables significant storage cost savings on FASTQ files. Fundamental features of the DRAGEN Platform address common challenges in genomic analysis, such as lengthy compute times and massive volumes of data.
Without compromising accuracy, the DRAGEN Platform delivers quickness, flexibility, and cost efficiency. The reprogrammable nature of the DRAGEN Platform enables Illumina to develop custom algorithms and allows for improvements to accommodate future applications.
|DRAGEN On-Premise Server||NextSeq 1000/2000||BaseSpace||Illumina Connected Analytics||Multi-Cloud Bring Your Own License|
|Pre-configured||Build your own|
|BCL Convert||Not recommended on FPGA|
|DRAGEN ORA compression|
|DRAGEN FASTQC + MultiQC|
|Whole genome||Germline + Somatic||Germline||Germline + Somatic||Germline + Somatic||Germline + Somatic||Germline + Somatic|
|Enrichment (including Exome)||Germline + Somatic||Germline + Somatic||Germline + Somatic||Germline + Somatic||Germline + Somatic||Germline + Somatic|
|NanoString GeoMx NGS|
|RNA Amplicon||Coming soon|
|RNA Pathogen Detection|
|COVID Lineage||Coming soon||Coming soon|
|TSO 500||ctDNA and Solid Coming soon for DRAGEN v3.10||ctDNA and Solid Coming soon for DRAGEN v3.10|
|PGx Star Allele Caller|
|Pipeline||Description||Variant Types Detected||Metrics Provided|
|DRAGEN Demultiplexing||Rapid demultiplexing of NGS analysis||N/A||N/A|
|DRAGEN Compression||DRAGEN ORA compression is optimized for high compression ratios of FASTQ files, as well as rapid compression and decompression, all while preserving data integrity.||N/A||
|DRAGEN Map + Align||The DRAGEN Map + Align can be run as a standalone or as part of DRAGEN’s suite of pipelines||N/A||
|DRAGEN Germline||The DRAGEN Germline Pipeline provides end-to-end NGS analysis, including advanced error model calibration for increased accuracy, and repeat expansion detection and genotyping through Illumina Expansion Hunter.||
|DRAGEN Somatic||The DRAGEN Somatic Pipeline includes tumor-only and tumor–normal modes, designed for detecting somatic variants in tumor samples. Both modes make no ploidy assumptions, enabling detection of low-frequency alleles.||
|DRAGEN Enrichment||The DRAGEN Enrichment Pipeline combines DRAGEN’s germline and somatic callers into a pipeline designed specifically for analyzing enrichment samples. Includes a full suite of enrichment metrics and reporting.||
|DRAGEN RNA||The DRAGEN RNA Pipeline performs transcriptome analysis starting with splice junction discovery and alignment, followed by rapid alignment and splice junction mapping and quantification. For differential expression, Illumina recommends the DRAGEN Differential Expression app on BaseSpace Sequence Hub.||
|DRAGEN Single Cell RNA||The DRAGEN Single Cell RNA pipeline performs demultiplexing, cell-barcode and UMI error correction, sequence alignment, and quantification of gene expression.||N/A||
|DRAGEN Joint Genotyping||The DRAGEN Joint Genotyping/Population Pipeline calls variants jointly across multiple genomes and scales to large cohorts of samples at expedited speeds with uncompromising accuracy.||
|DRAGEN Methylation||The DRAGEN Methylation Pipeline performs alignment, methyl calling, and calculates alignment and methylation metrics.||N/A||
|DRAGEN Reference Builder||Accepts FASTA files, and builds the proprietary reference used by the DRAGEN apps.||N/A||N/A|
|DRAGEN TruSight Oncology 500 ctDNA Analysis Software||Secondary analysis support for Illumina’s TruSight Oncology 500 ctDNA. Only available on the local DRAGEN Server (version 3)||
|DRAGEN Imputation||The DRAGEN Imputation pipeline is an end to end user friendly tool that enables scalable low pass whole genome sequencing analysis||N/A||Impute ≤100 samples simultaneously 1.7x faster compared to original GLIMPSE code|
Illumina is making it simpler for researchers to detect and identify SARS-CoV-2 and contribute their findings to the community with an integrated software toolkit, free of charge.Learn More
DRAGEN on Illumina Connected Analytics (ICA) couples the accuracy and speed of the DRAGEN with the ability to customize analysis pipeline to operationalize informatics on a secure platform.
DRAGEN on BaseSpace Sequence Hub (BSSH) provides push button analysis capability in an intuitive, easy-to-use interface with compliance, and storage features of BaseSpace Sequence Hub and Amazon Web Services (AWS).
DRAGEN on-premise offers highly accurate secondary analysis in a fraction of time compared with a traditional CPU-based system.
The on-board DRAGEN offers an easy to use, accurate, and efficient secondary analysis solution.
DRAGEN on AWS and Azure gives you access to DRAGEN software in a virtual image on your preferred cloud.
DRAGEN-GATK is a new best practices pipeline for secondary analysis of germline small variants, co-developed by the Broad Institute and Illumina. It combines the gold standard open-source GATK algorithms with the accuracy improvements from DRAGEN to ensure labs of all sizes and disciplines have access to high quality genomic analysis.View Webinar Recording
Basepaws, a Zoetis company leverages the Illumina DRAGEN Bio-IT Platform to develop direct-to-consumer DNA kits that provide cat owners with insights about their pets' health.Read Interview
Kyle Retterer, MS, Chief Technology Officer of GeneDx, discusses how their company used Azure to scale their computing and data storage. This evolution brought more data analysis speed, infrastructure security, risk management, and simplicity to their data management.Read Customer Spotlight
A rapid workflow with Illumina DNA PCR-Free Prep, NovaSeq 6000, and DRAGEN reduced turnaround time. The DRAGEN Platform was used to significantly increase the speed of mapping and aligning genomic data once sequenced.Read Article
The DRAGEN Platform can be used for numerous applications in the biological sciences.
Reduce time required for genomic analysis, with high accuracy and comprehensiveness
Analyze tumor-only and tumor/normal samples with accuracy, comprehensiveness, and efficiency
Advance understanding of cellular mechanisms with rapid analysis pipelines for bulk and single cell samples
Accurately and efficiently analyze sequenced genomes at scale. Accelerate re-analysis as computational tools improve over time
Detect and characterize infectious diseases with a comprehensive solution
Efficiently analyze animals and plants of varying genomic complexities with custom reference
DRAGEN provides the flexibility to insert a variety of input files and produce a range of output documents. This flexibility enables users to customize their experience and use and produce their desired file format.
This diagram shows steps in the DRAGEN Germline Pipeline as an example. The actual steps vary for each pipeline.