rRNA & Globin mRNA Removal Kit Selection Guide

Ribosomal RNA Depletion Selection Guide

Depletion of ribosomal RNA (rRNA) prior to RNA sequencing (RNA-Seq) enables researchers to focus on analyzing high-value portions of the transcriptome. Illumina Ribo-Zero rRNA Removal Kits and Globin-Zero rRNA/globin mRNA Removal Kits set the standard for rRNA and globin mRNA depletion for RNA-Seq library preparation.

The Ribo-Zero and Globin-Zero Kits:

  • Use intact, degraded and formalin-fixed, paraffin-embedded (FFPE) RNA samples.
  • Enrich for mRNA and non-rRNA non-coding RNA for whole transcriptome RNA-Seq.
  • Use an automation-friendly magnetic bead process.
  • Can be used with RNA from a wide variety of species.

RNA Sample Discontinued Kits Recommended Replacements Removes Euk. Cytoplasmic rRNA Removes Mitochondrial rRNA Removes Bacterial rRNA Removes Chloroplast rRNA Removes Globin mRNA
Human and other animal species and FFPE RNA Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat        
Human and other animal species; FFPE RNA Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) TruSeq Stranded Total RNA Gold      
Human microbiome and other bacteria-infected human, mouse, rat samples Ribo-Zero Gold rRNA Removal Kit (Epidemiology) Alternative solution pending    
Bacteria
(mixed populations of gram-negative and gram-positive bacteria)
Ribo-Zero rRNA Removal Kit (Bacteria) New product pending, contact Tech Support        
Gram-negative bacteria Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) New product pending, contact Tech Support        
Gram-positive bacteria Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) New product pending, contact Tech Support        
Plant leaf tissue Ribo-Zero rRNA Removal Kit (Plant Leaf) New product pending, contact Tech Support    
Plant seed or root tissue Ribo-Zero rRNA Removal Kit (Plant Seed/Root) TruSeq Stranded Total RNA Library Prep Plant    
Mammalian blood RNA Globin-Zero Gold Kit TruSeq Stranded Total RNA with Ribo-Zero Globin    
Yeast Ribo-Zero rRNA Removal Kit (Yeast) No alternative solutions