rRNA & Globin mRNA Removal Kit Selection Guide

Ribosomal RNA Depletion Selection Guide

Depletion of ribosomal RNA (rRNA) prior to RNA sequencing (RNA-Seq) enables researchers to focus on analyzing high-value portions of the transcriptome. Illumina rRNA removal solutions set the standard for rRNA and globin mRNA depletion for RNA-Seq library preparation.

The rRNA depletion process:

  • Works with intact, degraded, and formalin-fixed, paraffin-embedded (FFPE) RNA.
  • Enriches for mRNA and non-rRNA non-coding RNA for whole transcriptome RNA-Seq.
  • Features an automation-friendly magnetic bead process.
  • Can be utilized with RNA from a wide variety of species.

RNA Sample Discontinued Kits Recommended Replacements Removes Euk. Cytoplasmic rRNA Removes Mitochondrial rRNA Removes Bacterial rRNA Removes Chloroplast rRNA Removes Globin mRNA
Human and other animal species and FFPE RNA Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat        
Human and other animal species; FFPE RNA Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) TruSeq Stranded Total RNA Gold      
Human microbiome and other bacteria-infected human, mouse, rat samples Ribo-Zero Gold rRNA Removal Kit (Epidemiology) Alternative solution pending    
Bacteria
(mixed populations of gram-negative and gram-positive bacteria)
Ribo-Zero rRNA Removal Kit (Bacteria) New product pending, contact Tech Support        
Gram-negative bacteria Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria) New product pending, contact Tech Support        
Gram-positive bacteria Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) New product pending, contact Tech Support        
Plant leaf tissue Ribo-Zero rRNA Removal Kit (Plant Leaf) New product pending, contact Tech Support    
Plant seed or root tissue Ribo-Zero rRNA Removal Kit (Plant Seed/Root) TruSeq Stranded Total RNA Library Prep Plant    
Mammalian blood RNA Globin-Zero Gold Kit TruSeq Stranded Total RNA with Ribo-Zero Globin    
Yeast Ribo-Zero rRNA Removal Kit (Yeast) No alternative solutions      

Sample Type Discontinued Kits Recommended Replacements Input RNA (ScriptSeq) Comments (ScriptSeq)
Poly(A)-selected or rRNA-depleted RNA (user-provided) from any source, including FFPE tissue  ScriptSeq v2 RNA-Seq Library Preparation Kit TruSeq Stranded mRNA 500 pg - 50 ng of poly(A)-selected or rRNA-depleted RNA (user-provided) Does not include a Ribo-Zero rRNA or a Globin-Zero globin mRNA removal kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Human/Mouse/Rat and other higher vertebrates; FFPE RNA ScriptSeq Complete Gold Kit (Human/Mouse/Rat) TruSeq Stranded Total RNA 1 - 5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Blood ScriptSeq Complete Gold Kit (Blood) TruSeq Stranded Total RNA Globin 1 - 5 µg of total RNA Includes both a Globin-Zero rRNA/globin mRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Microbiome, bacterial infected human/mouse/rat ScriptSeq Complete Gold Kit (Epidemiology) Alternatives available from other suppliers 500 ng - 2.5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Bacteria ScriptSeq Complete Kit (Bacteria) Alternatives available from other suppliers 1 - 5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Plant leaf ScriptSeq Complete Kit (Plant Leaf) TruSeq Stranded Total RNA Plant 1 - 5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Plant seed or root ScriptSeq Complete Kit (Plant Seed/Root) TruSeq Stranded Total RNA Plant 1 - 5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).
Yeast ScriptSeq Complete Gold Kit (Yeast) Alternatives available from other suppliers 100 ng - 5 µg of total RNA Includes both a Ribo-Zero rRNA removal kit and ScriptSeq Library Prep kit. Requires FailSafe PCR Enzyme Mix (user-provided).