rRNA & Globin mRNA Removal Selection Guide 

Ribosomal RNA Depletion Selection Guide

Depletion of ribosomal RNA (rRNA) prior to RNA sequencing (RNA-Seq) allows researchers to focus on analyzing high-value portions of the transcriptome. Illumina rRNA removal solutions set the standard for rRNA and globin mRNA depletion for RNA-Seq library preparation.

The rRNA depletion process:

  • Works with intact, degraded, and formalin-fixed, paraffin-embedded (FFPE) RNA.
  • Enriches for mRNA and non-coding RNA for whole-transcriptome RNA-Seq.
  • Features an automation-friendly process.
  • Provides single-tube rRNA depletion of multiple species.

 

 Kit Kit Description RNA Sample Types Removes Euk. Cytoplasmic rRNA Removes Mitochondrial rRNA Removes Bacterial rRNA Removes Chloroplast rRNA Removes Globin mRNA Replaces Discontinued Kit
Illumina Stranded Total RNA Prep with Ribo-Zero Plus Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility. Human and other animal species; FFPE RNA  

Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat)

Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

Human/mouse/rat epidemiology (eg, virus-infected) and microbiome samples

  Ribo-Zero Gold rRNA Removal Kit (Epidemiology)
Bacteria
(mixed populations of gram-negative and gram-positive bacteria)
        Ribo-Zero rRNA Removal Kit (Bacteria)
Gram-negative bacteria (E. coli)

        Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria)
Gram-positive bacteria (Bacillus subtilis)         Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria)
Mammalian blood RNA   Globin-Zero Gold Kit
TruSeq Stranded Total RNA with Ribo-Zero Plant Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Plant leaf tissue

    Ribo-Zero rRNA Removal Kit (Plant Leaf)
Plant seed or root tissue     Ribo-Zero rRNA Removal Kit (Plant Seed/Root)
Custom solution Not currently available Yeast       Ribo-Zero rRNA Removal Kit (Yeast)
TruSeq Stranded Total RNA Gold A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue. Human and other animal species and FFPE RNA      
TruSeq Stranded Total RNA with Ribo-Zero Globin Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step. Mammalian blood RNA