The product configuration for Cat. No. RS-122-2501, RS-122-2502, and RS-122-2503 has changed. In the new configuration, the library preparation and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.Read More...
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Library Prep

TruSeq® Stranded Total RNA Library Prep Globin (48 Samples)

20020612

Price
 
 

TruSeq® Stranded Total RNA Library Prep Globin (96 Samples)

20020613

Price
 
 

Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

Price
 
 

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

Price
 
 

Product Highlights

TruSeq Stranded Total RNA with Ribo-Zero Globin delivers a clear and comprehensive view of the transcriptome from blood-derived total RNA, with fast, efficient sequencing library preparation.

High-quality whole-transcriptome analysis with precise strand information

These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

  • Uniform coverage and precise measurement of strand orientation
  • High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
  • Discovery across both coding and multiple forms of noncoding RNA
Efficient ribosomal RNA and globin mRNA removal in a single step

Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

Traditional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Automation-friendly workflow

Find automation vendors with robotic systems compatible with this product

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
2 x 75 bp
2 x 75 bp
HiSeq 2500 System RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample)
Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample)
2 x 75 bp
2 x 75 bp
NovaSeq 6000 System RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. 
≤ 2 × 100 bp
≤ 2 × 100 bp

Product Comparison

TruSeq Stranded Total RNA with Ribo-Zero Globin TruSeq Stranded Total RNA AmpliSeq for Illumina Transcriptome Human Gene Expression Panel
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA > 95% of human RefSeq genes; 20,802 genes; captures human transcriptome
Description For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. Study the expression levels of >20,000 human RefSeq genes using sequence-specific amplicons.
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. 1-100 ng RNA (10 ng recommended)
Method Whole-Transcriptome Sequencing Whole-Transcriptome Sequencing Exome Sequencing , mRNA Sequencing , Targeted RNA Sequencing
Specialized Sample Types Blood FFPE Tissue Blood, FFPE Tissue
Species Category Human, Mouse, Rat Human, Mouse, Rat Human

Method-Specific Workflow Example

 

Supporting Data and Figures

 

Related Products

Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Globin Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.