The product configuration for Cat. No. RS-122-2401, RS-122-2402, and RS-122-2403 has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA with Ribo-Zero Plant

Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.Read More...
Select Product(s)
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Library Prep

TruSeq® Stranded Total RNA Library Prep Plant (48 Samples)

20020610

Price
 
 

TruSeq® Stranded Total RNA Library Prep Plant (96 Samples)

20020611

Price
 
 

Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

Price
 
 

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

Price
 
 

Product Highlights

High-Quality Whole-Transcriptome Analysis with Precise Strand Information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression across both coding and multiple forms of noncoding RNA. TruSeq Stranded Total RNA with Ribo-Zero Plant couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find automation vendors with robotic systems compatible with this product

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs

TruSeq Stranded Total RNA with Ribo-Zero Plant enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. These products have been validated for use in multiple plant species, including Arabidopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) 2 x 75 bp
NextSeq 550 System Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) 2 x 75 bp
HiSeq 2500 System RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample) 2 x 75 bp
HiSeq 2500 System Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) 2 x 75 bp
NovaSeq 6000 System RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. ≤ 2 × 100 bp
NovaSeq 6000 System Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.  ≤ 2 × 100 bp

Method-Specific Workflow Example

 

Customer Stories

Next-Generation Sequencing Saves the Sweet Potato

Researchers at the ARC of South Africa use the MiSeq System to identify and map sweet potato viruses, with the goal of enhancing food security in developing countries.

Learn More

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Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Plant Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc.  The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity).  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.