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Advanced chemistry, optics, and informatics combine to deliver exceptional speed and data quality, outstanding throughput and scalability.
TruSeq Stranded Total RNA with Ribo-Zero Plant
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.
The product configuration for Cat. No. RS-122-2401, RS-122-2402, and RS-122-2403 has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
Overview
High-quality whole-transcriptome analysis with precise strand information
RNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression across both coding and multiple forms of noncoding RNA. TruSeq Stranded Total RNA with Ribo-Zero Plant couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.
Efficient ribosomal RNA reduction across a range of plant species, study designs
TruSeq Stranded Total RNA with Ribo-Zero Plant enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. These products have been validated for use in multiple plant species, including Arabidopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.
Specifications
| Automation capability | Liquid handling robots |
|---|---|
| Content specifications | Captures coding RNA plus multiple forms of non-coding RNA |
| Input quantity | 0.1 – 1 ug high-quality total RNA extracted from plant samples |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NovaSeq X System, NextSeq 500 System, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | Bead-based rRNA depletion, cDNA synthesis, and PCR |
| Method | Whole-transcriptome sequencing |
| Multiplexing | Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together, High-throughput kits: Prepare 96 indexed samples using either combinatorial dual indexes or unique dual indexes |
| Nucleic acid type | RNA |
| Species category | Plant |
| Technology | Sequencing |
| Variant class | Single nucleotide polymorphisms (SNPs), Gene fusions, Novel transcripts, Transcript variants |
Required Products
Illumina now offers modular product ordering to enable flexibility in your workflows. You may order any of the following library prep products with any of the following indexes.
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Results
Applications
Example workflow
Project recommendations
| Instrument | Recommended number of samples | Read length |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per sample) |
2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads. |
≤ 2 × 100 bp |
Related applications and methods
Genome sequencing in agriculture can help improve our food supply, ensure a sustainable environment, and support species conservation.
RNA-Seq uses next-generation sequencing to analyze expression across the transcriptome, enabling scientists to detect known or novel features and quantify RNA.
Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
How others use this product
Documentation
Support documentation
- TruSeq Stranded Total RNA Support - Documentation & Literature Documentation
- TruSeq Stranded Total RNA Consumables & Equipment Documentation
- TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation
- TruSeq Stranded Total RNA Documentation
- TruSeq Stranded Total RNA Reference Guide Documentation
- TruSeq Stranded Total RNA Checklist Documentation
- All TruSeq Stranded Total RNA Support
Library prep (2)
TruSeq® Stranded Total RNA Library Prep Plant (48 Samples)
20020610
Includes RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast rRNA from plant samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.
List Price:
Discounts:
TruSeq® Stranded Total RNA Library Prep Plant (96 Samples)
20020611
Includes RiboZero beads to deplete cytoplasmic, mitochondrial, and chloroplast rRNA from plant samples, PCR master mix to transcript RNA into cDNA, and reagents for preparing 48 samples. Purchase index adapters and Purification Beads separately.
List Price:
Discounts:
Index adapters (3)
IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)
20020591
New Unique Dual Indexes (UDIs), compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. The UDI kit is provided in a plate format, contains 24 UDIs (x4), sufficient for 96 samples.
IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)
20040870
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Nano and TruSeq PCR-Free Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022370, with no changes to the remaining 184. Purchase library preps and probe panels separately.
List Price:
Discounts:
IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)
20040871
Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA Library Prep Kits. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022371, with no changes to the remaining 184. Purchase library preps and probe panels separately.
List Price:
Discounts:
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Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
TruSeq Stranded Total RNA
A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
TruSeq Stranded mRNA
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
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