The product configuration for Cat. No. RS-122-2401, RS-122-2402, and RS-122-2403 has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA with Ribo-Zero Plant

Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.Read More...
Select Product(s)
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Library Prep

TruSeq® Stranded Total RNA Library Prep Plant (48 Samples)



TruSeq® Stranded Total RNA Library Prep Plant (96 Samples)



Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)



TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)



TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)



IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)



IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)



Product Highlights

High-Quality Whole-Transcriptome Analysis with Precise Strand Information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation, and high-confidence discovery of features such as alternative transcripts, antisense expression, and allele-specific expression across both coding and multiple forms of noncoding RNA. TruSeq Stranded Total RNA with Ribo-Zero Plant couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

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Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs

TruSeq Stranded Total RNA with Ribo-Zero Plant enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. These products have been validated for use in multiple plant species, including Arabidopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.


Method-Specific Workflow Example


Customer Stories

Next-Generation Sequencing Saves the Sweet Potato

Researchers at the ARC of South Africa use the MiSeq System to identify and map sweet potato viruses, with the goal of enhancing food security in developing countries.

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Product Literature

Manuals and Support Information

TruSeq Stranded Total RNA Support - Documentation & Literature

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Stranded Total RNA Support 

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Limited Use Label License: The TruSeq Stranded Total RNA with Ribo-Zero Plant Workflow product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc.  The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity).  The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product.  The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERCIAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.