This kit is compatible with unique dual index kits (available soon), which allow multiplexing of up to 96 samples.

TruSeq Stranded Total RNA Library Prep Kit

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and FFPE. Read More...
Select Product(s)

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set A (48 samples, 12 indexes)

RS-122-2201

Price
 
 

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set B (48 samples, 12 indexes)

RS-122-2202

Price
 
 

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat High Throughput (96 samples, 96 indexes)

RS-122-2203

Price
 
 

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (48 samples, 12 indexes)

RS-122-2301

Price
 
 

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set B (48 samples, 12 indexes)

RS-122-2302

Price
 
 

TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)

RS-122-2303

Price
 
 

Product Highlights

The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compatible with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

Flexible Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
  • TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
  • The total RNA kits are also compatible with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications

Method-Specific Workflow Example

 

Customer Stories

BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

Read More

Supporting Data and Figures

 

Product Literature

Manuals and Support Information

TruSeq Stranded Total RNA LT Kit Documentation  

TruSeq Stranded Total RNA HT Kit Documentation  

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All TruSeq Stranded Total RNA LT Kit Support 

All TruSeq Stranded Total RNA HT Kit Support 

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Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.