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TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue. Read More...
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Library Prep

TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples)

20020596

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TruSeq® Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)

20020597

Price
 
 

TruSeq Stranded Total RNA Library Prep Gold (48 Samples)

20020598

Price
 
 

TruSeq® Stranded Total RNA Library Prep Gold (96 Samples)

20020599

Price
 
 
Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

Price
 
 

TruSeq RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)

20040871

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

Price
 
 
Services

TruSeq Total RNA Training

15044069

Accessory Products

Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

Price
 
 

Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

Price
 
 

Illumina Ribo-Zero plus rRNA Depletion Kit (96 Samples)

20037135

Price
 
 
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TruSeq Stranded Total RNA

Product Highlights

TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Species Compatibility

TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.

View species compatibility tables

Flexible Solutions for a Range of Study Designs

TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA Human/Mouse/Rat removes cytoplasmic rRNA
  • TruSeq Stranded Total RNA Gold removes both cytoplasmic and mitochondrial rRNA
  • The total RNA kits are also compatible with the new unique dual (UD) index kits, IDT for Illumina - TruSeq RNA UD Indexes, which allows multiplexing of up to 96 samples

Find automation vendors with compatible robotic systems

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)		 2 x 75 bp
HiSeq 2500 System RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample)
Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample)		 2 x 75 bp
NovaSeq 6000 System RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. 		 ≤ 2 × 100 bp

Product Comparison

TruSeq Stranded Total RNA Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome
Assay Time 11.5 hours ~7 hours
Automation Capability Liquid Handling Robots Liquid Handling Robots
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA Captures coding RNA plus multiple forms of non-coding RNA
Description For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. For whole-transcriptome sequencing studies of multiple sample types. Includes Ribo-Zero plus module for single-tube depletion of abundant transcripts from multiple species
Hands-On Time 5.5 hours < 3 hours
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. 1-1000 ng total RNA for standard-quality RNA samples. 10ng total RNA minimum recommended for optimal performance and FFPE samples
Mechanism of Action Bead-based rRNA depletion, cDNA synthesis, and PCR Enzymatic rRNA depletion, ligation-based addition of adapters and indexes
Method Whole-Transcriptome Sequencing Metatranscriptome Sequencing, Whole-Transcriptome Sequencing
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together, High-throughput kits: Prepare 96 indexes samples using either combinatorial dual indexes or unique dual indexes (available soon) Up to 384 Unique Dual Indexes (UDIs)
Specialized Sample Types FFPE Tissue
Species Category Human, Mouse, Rat Bacteria, Human, Mouse, Rat
Species Details rRNA Removal: Depletes human, mouse, and rat rRNA Ribo-Zero Plus included for depletion of abundant transcripts from multiple species including: human cytoplasmic & mitochondria rRNA, mouse rRNA, rat rRNA, bacteria Gram +/- rRNA, human beta globin transcripts
Technology Sequencing Sequencing

The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

Method-Specific Workflow Example

 
Prepare Library for:
Total RNA Sequencing
 
Sequence Efficiently with:
NextSeq 550 System
NovaSeq 6000 System
 
Analyze Your Data with:
DRAGEN RNA
BaseSpace RNA-Seq Differential Expression App
BaseSpace RNA Express App
 
 

Supporting Data and Figures

 

Product Literature

Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold

Application Note | HTML

Data Sheet | PDF | 5 versions

Manuals and Support Information

TruSeq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA Depletion Reference Guide Documentation

TruSeq Stranded Total RNA Reference Guide Documentation

TruSeq Stranded Total RNA Checklist Documentation

TruSeq Stranded Total RNA Documentation

TruSeq Stranded Total RNA Consumables & Equipment Documentation

TruSeq Stranded Total RNA Support - Documentation & Literature

Custom Protocol Selector
Generates customized, end-to-end instructions

All TruSeq Stranded Total RNA Support  

Find the Right Kit

Determine the best library prep kit or array for your needs based on your starting material and method of interest.

Access Tool
Find the Right Kit

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Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.

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Not for use in diagnostic procedures (except as specifically noted).

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