|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 6-20 samples per run (based on 20 million reads per
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
|2 x 75 bp|
|HiSeq 2500 System||RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample)
Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample)
|2 x 75 bp|
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
|≤ 2 × 100 bp|
|TruSeq Stranded Total RNA||TruSeq Stranded Total RNA with Ribo-Zero Globin|
|Content Specifications||Captures coding RNA plus multiple forms of non-coding RNA||Captures coding RNA plus multiple forms of non-coding RNA|
|Description||For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA.||For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA.|
|Input Quantity||0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.||0.1 – 1 ug high-quality purified total RNA from blood|
|Method||Whole-Transcriptome Sequencing||Whole-Transcriptome Sequencing|
|Specialized Sample Types||FFPE Tissue||Blood|
|Species Category||Human, Mouse, Rat||Human, Mouse, Rat|
BaseSpace Sequence Hub SRA Submission App enables tracking of samples, from library prep to data analysis, helping the Biotechnology Resource Center Sequencing Core (BRC-Seq) at the University of British Columbia deliver high-quality sequencing data and additional value to its customers.Read More
Consistent, precise measurement of RNA abundance is reflected by high reproducibility between technical replicates. These technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverge of combined reads.
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