The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.

TruSeq Stranded Total RNA

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue. Read More...
Select Product(s)
What products do I need?
Library Prep

TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (48 Samples)

20020596

Price
 
 

TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat (96 Samples)

20020597

Price
 
 

TruSeq® Stranded Total RNA Library Prep Gold (48 Samples)

20020598

Price
 
 

TruSeq Stranded Total RNA Library Prep Gold (96 Samples)

20020599

Price
 
 

Index Adapters

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

Price
 
 

TruSeq® RNA Single Indexes Set A (12 Indexes, 48 Samples)

20020492

Price
 
 

TruSeq® RNA Single Indexes Set B (12 Indexes, 48 Samples)

20020493

Price
 
 

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

Price
 
 

Product Highlights

TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE), TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Species Compatibility

TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information about a particular species of interest, contact Illumina Technical Support.

Flexible Solutions for a Range of Study Designs

TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:

  • TruSeq Stranded Total RNA Human/Mouse/Rat removes cytoplasmic rRNA
  • TruSeq Stranded Total RNA Gold removes both cytoplasmic and mitochondrial rRNA
  • The total RNA kits are also compatible with the new unique dual (UD) index kits, IDT for Illumina - TruSeq RNA UD Indexes, which allows multiplexing of up to 96 samples

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this workflow

96 UD Indexes available soon

Specifications

Method-Specific Workflow Example

 

Customer Stories

BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace Sequence Hub SRA Submission App enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

Read More

Supporting Data and Figures

 

Related Products

Image Source
TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.


Image Source
TruSeq Stranded Total RNA with Ribo-Zero Plant

Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.


Image Source
TruSeq Stranded mRNA

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.


Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.