The products previously known as TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Cat. No. RS-122-2201, RS-122-2202, and RS-122-2203) and TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Cat. No. RS-122-2301, RS-122-2302, and RS-122-2303) are now called TruSeq Stranded Total RNA Human/Mouse/Rat and TruSeq Stranded Total RNA Gold, respectively. The product configuration has changed. In the new configuration, the library preparation reagents and index adapters can be purchased separately. The list of components available for individual purchase can be found in the “Select Product(s)” section.
TruSeq Stranded Total RNA provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
Compatible with a wide range of samples, including low-quality DNA/RNA and formalin-fixed, paraffin-embedded (FFPE) samples, TruSeq Stranded Total RNA couples all of the benefits of TruSeq RNA technology with Ribo-Zero ribosomal RNA reduction chemistry. The product enables analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
TruSeq Stranded Total RNA is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*
*For information about a particular species of interest, contact Illumina Technical Support.
TruSeq Stranded Total RNA is available in multiple versions to fit specific study design needs:
Find automation vendors with robotic systems compatible with this product
Read a comprehensive assessment of Illumina’s ribosomal RNA depletion protocol
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | RNA Profiling: 6-20 samples per run (based on 20 million reads per
sample) Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample) |
2 x 75 bp |
| HiSeq 2500 System | RNA Profiling: 30-200 samples per run (dual flow cell; based on 20
million reads per sample) Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample) |
2 x 75 bp |
| NovaSeq 6000 System | RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4:
768. Limited by index combinations (dual). Based on 20 million reads. Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads. |
≤ 2 × 100 bp |
| TruSeq Stranded Total RNA | TruSeq Stranded Total RNA with Ribo-Zero Globin | |
|---|---|---|
| Content Specifications | Captures coding RNA plus multiple forms of non-coding RNA | Captures coding RNA plus multiple forms of non-coding RNA |
| Description | For whole-transcriptome sequencing studies. The Total RNA version depletes cytoplasmic rRNA while the Total RNA Gold version depletes cytoplasmic and mitochondrial rRNA. | For whole-transcriptome sequencing studies of blood-derived RNA. Depletes cytoplasmic and mitochondrial rRNA plus globin mRNA. |
| Input Quantity | 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization. | 0.1 – 1 ug high-quality purified total RNA from blood |
| Method | Whole-Transcriptome Sequencing | Whole-Transcriptome Sequencing |
| Specialized Sample Types | FFPE Tissue | Blood |
| Species Category | Human, Mouse, Rat | Human, Mouse, Rat |
BaseSpace Sequence Hub SRA Submission App enables tracking of samples, from library prep to data analysis, helping the Biotechnology Resource Center Sequencing Core (BRC-Seq) at the University of British Columbia deliver high-quality sequencing data and additional value to its customers.
Read More
Consistent, precise measurement of RNA abundance is reflected by high reproducibility between technical replicates. These technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverge of combined reads.
Robust low-input RNA sequencing with TruSeq Stranded Total RNA Library Prep Gold
Application Note | HTML
Data Sheet | PDF | 5 versions
TruSeq Stranded Total RNA Support - Documentation & Literature
TruSeq Stranded Total RNA Consumables & Equipment Documentation
TruSeq Stranded Total RNA Documentation
TruSeq Stranded Total RNA Checklist Documentation
TruSeq Stranded Total RNA Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
Determine the best library prep kit or array for your needs based on your starting material and method of interest.
Access Tool
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes ribosomal RNA and globin mRNA in a single step.
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep workflow. Remove rRNA from plant leaf, seed, and root tissue.
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
