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This kit is compatible with unique dual index kits (available soon), which allow multiplexing of up to 96 samples.
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set A (48 samples, 12 indexes)
RS-122-2201
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set B (48 samples, 12 indexes)
RS-122-2202
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat High Throughput (96 samples, 96 indexes)
RS-122-2203
TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (48 samples, 12 indexes)
RS-122-2301
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.
Compatible with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*
*For information on a particular species of interest, contact Illumina Technical Support.
BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.
Read More
Consistent, precise measurement of RNA abundance is reflected by high reproducibility between technical replicates. These technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverge of combined reads.
TruSeq Stranded mRNA and Total RNA Library Preparation Kits
Brochure | PDF(8 MB)
TruSeq Stranded Total RNA LT Kit Documentation
TruSeq Stranded Total RNA HT Kit Documentation
Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.
Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes rRNA and globin mRNA in a single step.
Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep kit. Remove rRNA from plant leaf, seed, and root tissue.
Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.
Limited Use Label License: This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft, exclusively licensed to New England Biolabs, Inc. and sublicensed to Illumina, Inc. The purchase of this product from Illumina, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product by the buyer (whether the buyer is an academic or for profit entity). The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications direct to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use the product for the following COMMERICAL PURPOSES: (I) use of the product or its components in manufacturing; or (2) use of the product or its components for therapeutic or prophylactic purposes in human or animals.
