TruSeq Total RNA Training
|Instrument||Recommended Number of Samples||Read Length|
|NextSeq 550 System||RNA Profiling: 6-20 samples per run (based on 20 million reads per sample)
Transcriptome Analysis: 2-8 samples per run (based on 50 million reads per sample)
|2 x 75 bp|
|HiSeq 2500 System||RNA Profiling: 30-200 samples per run (dual flow cell; based on 20 million reads per sample)
Transcriptome Analysis: 12-80 samples per run (dual flow cell; based on 50 million reads per sample)
|2 x 75 bp|
|NovaSeq 6000 System||RNA Profiling (samples per run, dual flow cell): S1: 160, S2: 320, S4: 768. Limited by index combinations (dual). Based on 20 million reads.
Transcriptome Analysis (samples per run, dual flow cell): S1: 64, S2: 128, S4: 400. Limited by index combinations (dual). Based on 50 million reads.
|≤ 2 × 100 bp|
|TruSeq Stranded Total RNA||Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome|
|Automation Capability||Liquid Handling Robots||Liquid Handling Robots|
|Method||Whole-Transcriptome Sequencing||Metatranscriptome Sequencing, Whole-Transcriptome Sequencing|
|Specialized Sample Types||FFPE Tissue|
|Species Category||Human, Mouse, Rat||Bacteria, Human, Mouse, Rat|
BaseSpace Sequence Hub helps the Biotechnology Resource Center Sequencing Core (BRC-Seq) at the University of British Columbia deliver high-quality sequencing data and additional value to its customers.Read More
Consistent, precise measurement of RNA abundance is reflected by high reproducibility between technical replicates. These technical replicates of FFPE tissue show high concordance, indicating robust library prep performance. Axes are log2(FPKM). R2 value is shown.
TruSeq Stranded Total RNA gives excellent coverage across the top 1000 expressed transcripts in both fresh-frozen (FF, top) and FFPE (bottom) tumor and matched normal breast tissue, with > 98% aligned stranded reads. X-axis: position along transcript, Y-axis = percent coverge of combined reads.
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