THS-Seq
THS-Seq
THS-seq is a method for highly sensitive characterization of chromatin accessibility. This variation on ATAC-seq uses linear amplification of accessible DNA ends, in vitro transcription, and an engineered Tn5 super-mutant.
Pros:
- Requires very little input materials
- Can detect small regions near distal enhancers
Cons:
- Requires an engineered transposase