Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in less than 90 minutes, with a low DNA input requirement. Read More...
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Nextera XT DNA Library Preparation Kit (24 samples)



Nextera XT DNA Library Preparation Kit (96 samples)


Accessory Products
What accessories do I need?

Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)



Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)



Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)



Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)



Nextera XT Index Kit (24 indexes, 96 samples)



Nextera® XT Index Kit (96 indexes, 384 samples)



Product Highlights

Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
  • Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
  • Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
  • Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
  • Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit

When to Use Standard vs. Bead-Based Normalization

For high-throughput researchers, bead-based normalization is an option that saves time and resources by providing an accelerated path from DNA to data. This technical note provides guidelines and data comparing the two methods to assist you in deciding which option to use.

View Tech Note
When to Use Standard vs. Bead-Based Normalization


Method-Specific Workflow Example


Customer Stories

Mapping Neural Diversity: A Molecular Analysis of Neuronal Identity in Mouse Primary Visual Cortex

Single-cell mRNA-Seq with the MiSeq and HiSeq Systems is enabling Allen Institute researchers to classify V1 neural cells.

Read More
Next-Generation Sequencing Aids Researchers in the Fight Against the Ebola Virus

Genomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the virus’s rapid mutation rate.

Read More

Supporting Data and Figures


Manuals and Support Information

Nextera XT DNA Library Prep Kit Documentation

Custom Protocol Selector
The Custom Protocol Selector enables you to generate your own customized documentation, specifically tailored to your experiment.

All Nextera XT DNA Library Prep Kit Support 

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