Processing more samples in less time

Sample multiplexing exponentially increases the number of samples sequenced per run

Multiplex Sequencing

Multiplex sequencing allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on a high-throughput instrument. Sample multiplexing is useful when targeting specific genomic regions or working with smaller genomes.

Individual "barcode" sequences are added to each DNA fragment during next-generation sequencing (NGS) library preparation so that each read can be identified and sorted before the final data analysis. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.

Conceptual Overview of Sample Multiplexing
  • Fast High-Throughput Strategy: Large sample numbers can be simultaneously sequenced during a single experiment
  • Cost-Effective Method: Sample pooling improves productivity by reducing time and reagent use
  • High-Quality Data: Accurate maintenance of read length of unknown sequences
  • Simplified Analysis: Automatic sample identification with "barcodes" using Illumina data analysis software
Multiplexing Facilitates Microbial Studies

Researchers leverage the speed and multiplexing capabilities of Illumina sequencers to identify microbes along the Mississippi River.

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  • Sequencing: Ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Explore a broad range of sequencing methods.
  • High-Throughput Library Prep Automation: Learn about automated liquid handling solutions designed to help labs prepare large quantities of sequencing libraries.
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Flexible High-Throughput Sequencer

The NovaSeq 6000 System offers scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.

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Chan Zuckerberg Biohub and the NovaSeq System

Researchers at the Chan Zuckerberg Biohub are working to conduct innovative experiments and form new collaborations in genomics. The NovaSeq System enables them to bundle many samples together for medical research that may help to improve human health.

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Chan Zuckerberg Biohub and the NovaSeq System
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