Processing more samples in less time

Sample multiplexing exponentially increases the number of samples sequenced per run

Multiplex Sequencing

Process a large number of samples with multiplex sequencing on a high-throughput instrument. Sample multiplexing is a useful technique when targeting specific genomic regions or working with smaller genomes.

To accomplish this, individual "barcode" sequences are added to each sample so they can be distinguished and sorted during data analysis. Pooling samples exponentially increases the number of samples analyzed in a single run, without drastically increasing cost or time.

Conceptual Overview of Sample Multiplexing
  • Fast, High-Throughput Strategy: Large sample numbers can be simultaneously sequenced during a single experiment
  • Cost-Effective Method: Multisample pooling improves productivity by reducing time and reagent use
  • High-Quality Data: Accurate maintenance of read length of unknown sequences
  • Simplified Analysis: Automatic sample identification with "barcodes" using Illumina data analysis software
Explore the NovaSeq Series

Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.

Learn More
Interested in receiving newsletters, case studies, and information from Illumina based on your area of interest? Sign up now.