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Tagmentation

Reduce your library prep time with on-bead tagmentation

Novel chemistry integrates DNA extraction, fragmentation, library prep, and library normalization

What is Tagmentation?

Tagmentation is the initial step in library prep where unfragmented DNA is cleaved and tagged for analysis.

On-bead tagmentation library prep uses bead-linked transposomes for a more uniform tagmentation reaction compared to in-solution tagmentation reactions. Bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization steps. This reduces the number of workflow steps, requiring low sample input and reducing both hands-on and turnaround time.

How On-Bead Tagmentation Works

See how bead-linked transposome technology integrates and reduces workflow steps.

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Why On-Bead Tagmentation is Better

The benefits of bead-linked transposome library prep over in-solution tagmentation include:

  • Low sample input requirements
  • Library quantitation and normalization steps eliminated, result in shorter turnaround and reduced hands-on time
  • On-bead fragmentation removes the need for DNA shearing
  • Uniform and consistent insert sizes and library yields across a wide DNA input range

On-bead tagmentation can reduce your library preparation time, while delivering consistent insert sizes, uniform coverage, and optimized performance, regardless of the DNA input amount or genome size.

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Flexibility for Your Experiments

On-bead tagmentation library prep has a wide DNA input range that provides flexibility for experiments with various sample types, including precious samples. This technology is ideal for applications such as human whole-genome sequencing, cancer genomics research, environmental metagenomics, infectious disease research, agrigenomics, and more with:

  • DNA whole-genome sequencing of any species, large or small
  • Targeted DNA enrichment applications without unique molecular identifiers, including whole exome sequencing, fixed panels, custom panels, and Illumina and third-party oligo vendors
  • RNA enrichment
A Novel Library Prep Technology

Learn how Illumina DNA Prep (formerly Nextera DNA Flex) uses a novel on-bead tagmentation technology to retain the convenience of enzymatic fragmentation while addressing the shortcoming of enzymatic fragmentation.

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Frequently Purchased Together

Choosing a Tagmentation Kit for Your Experiment

Applications Product Benefits
16s rRNA Sequencing, Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Nextera XT
  • Optimized for research on small genomes, PCR amplicons, and plasmids
Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Illumina DNA Prep
  • No library quantification needed
Human Whole-Genome Sequencing Illumina DNA PCR-Free
  • Bead-linked transposome technology
  • Avoid PCR duplicates
  • No library quantification needed

Comparing Nextera and TruSeq Technologies

Nextera uses bead-linked transposome technology. Integrated DNA extraction, automatic fragmentation, library preparation, and library normalization are included in the protocol. It’s ideal for whole-genome sequencing and target enrichment applications.

TruSeq uses adapter ligation, fragmentation, end repair, and has Poly(A) capture efficiency. It requires separate DNA shearing. It captures the coding transcriptome and is ideal for whole transcriptome RNA-Seq, exome enrichment, large-scale whole-genome resequencing, targeted resequencing, de novo sequencing, metagenomics, and methylation studies.

Learn More About Adapter Ligation

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Other Technologies to Meet Your Needs

Automation

Our partners have developed both high- and low-throughput walk-away automation methods that span our library prep portfolio.

Index Adapters

Increase the number of samples sequenced per run and optimize high-throughput sequencing using unique dual index adapters.

UMIs

Unique molecular identifiers (UMIs) provide error correction and accuracy. They can reduce false-positive variant calls while increasing variant detection sensitivity.