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Library Prep Automation

Automation Methods that Span Our Library Prep Portfolio

Minimize errors, reduce hands-on time, and enable higher throughput

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Automate Your Library Prep

Reduce hands-on time with library prep automation. Automating your library prep minimizes errors and is available for a range of throughput and capital expenditure needs. The liquid handling robots used for automation can be programmed for various lab needs, making them ideal for labs that require high flexibility.

Our automation partners have developed automation methods that span our library prep portfolio. They offer both high- and low-throughput walk-away automation methods. The Illumina-Qualified designation means we’ve performed analyses that show libraries prepared with these methods perform comparably to libraries prepared manually.

We make it easy to automate your library prep. The vendors below have developed automated methods for Illumina kits, eliminating the time and effort needed to develop your own method.

  Agilent Beckman Coulter Eppendorf Hamilton PerkinElmer Tecan
16S rRNA     epMotion 5075t
epMotion 5073
NGS STAR    
AmpliSeq for Illumina*   Biomek i5 epMotion 5075t
epMotion 5073
NGS STAR
NGS STARlet
Scicione  
Nextera DNA Exome Bravo Option B          
Illumina DNA Prep Bravo Option B Biomek FXp
Biomek iSeries
  NGS STAR Scicione Freedom EVO NGS
Illumina DNA Prep with Enrichment   Biomek FXp epMotion 5075t NGS STAR Scicione Freedom EVO NGS
Nextera XT DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione Freedom EVO NGS
TruSeq DNA PCR-Free Bravo Option B Biomek FXp epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq Nano DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq RNA Exome Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq RNA Fusion     epMotion 5075t      
TruSeq RNA Pan Cancer            
TruSeq RNA v2   Biomek FXp     Scicione NGS and NGSx  
TruSeq Stranded mRNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSeq Stranded Total RNA Bravo Option B Biomek FXp epMotion 5075t NGS STAR Scicione NGS and NGSx Freedom EVO NGS
TruSight Oncology 500   Biomek FXp
Biomek i5
  NGS STAR    
TruSight Tumor 15   Biomek 4000 epMotion 5075t Zephyr    
TruSight Tumor 170   Biomek NXp   NGS STAR    

Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)

Documentation: user guide and / or app note available

*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.

Frequently Purchased Together

Automated Library Prep Workflow Examples

Assessing Nucleic Acid Quality with Fragment Analyzer™

The Fragment Analyzer offers automated fragmentation and size selection. It accurately assesses quality and quantity of prepared libraries to optimize cluster generation during sequencing, and it accommodates both low- and high-throughput labs.

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Automated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics

The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.

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Library Prep Technical Tips

DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.