Library Preparation
DNA Sequencing RNA Sequencing Methylation Sequencing Library Preparation High-Throughput Sequencing

Library prep automation

Minimize errors, reduce hands-on-time, and enable higher throughput with automation options across Illumina library prep products

Scientist starting library prep automation sequence

Automate your library prep

Automated liquid-handling systems help labs achieve and maintain consistent results while driving efficiency. NGS library prep automation also allows for the rapid scaling of throughput while reducing hands-on time.

Illumina partners with leading vendors to provide automated protocols for Illumina library prep kits, combining our partners’ automation knowledge with our library prep and sequencing expertise to build an NGS solution that works for you.

Accelerate your NGS workflow with Illumina-ready automation

Integrated workflows

Illumina offers components for every stage of the next-generation sequencing (NGS) process—from library prep and automation protocols to sequencing, data analysis, and support.

Flexible throughput options

Configure automation setups to meet a range of sample volumes and workflow needs.

Access to Illumina-ready protocols

Use protocols developed and qualified in collaboration with automation partners alongside Illumina library prep kits.

Comprehensive support

Get help with setup, training, and ongoing technical support.

Library Prep Automation Buyer’s Guide

This guide walks you through the range of automation tools available for NGS library prep and helps you find a solution tailored for your application, throughput, and budget needs. Learn about top considerations for building your automated library prep protocol.

Featured Illumina-ready protocols

TruSight Oncology 500 v2

Enable CGP with a large pan-cancer panel covering all main variant classes plus key biomarkers (TMB, MSI, and HRD) from FFPE tissue.

TruSight Oncology 500 ctDNA v2

Enable CGP with a pan-cancer panel capable of detecting key IO gene signatures (TMB, MSI) plus all main variant classes from ctDNA in blood plasma.

Flexible automation, the way you need it

Choose between Illumina-ready, end-to-end support or flexible partner-developed solutions—both designed to streamline NGS library prep from DNA to results.

Full Illumina-ready automation support

Best for teams that want Illumina-ready protocols and direct Illumina support.

  • Validated protocols
    Co-developed and qualified with Illumina
  • Confidence in training
    Illumina-led onboarding for your team
  • Production-ready
    Illumina assists with performance qualification
  • Direct support
    Illumina is your main technical contact
  • Hardware-backed
    Automation partners service their systems

Illumina partner network

Best for teams that want flexibility with partner-developed workflows, with Illumina as backup.

  • Partner-developed protocols

    Certified by Illumina if assay metrics are met
  • Partner-managed support
    Install, service, and maintain systems
  • Field expertise
    Partners provide training and performance qualification
  • Illumina backup
    Secondary support for chemistry when needed
  • Flexibility
    Broader choice of platforms and providers

All Illumina automation protocols

Determine which library prep kits are automated on your liquid handling platform of interest. Find Illumina-ready and/or partner-developed solutions.

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Automation platform info and documentation

Illumina partner documentation

Learn more about the automated liquid-handling platforms offered by Illumina partners. View partner documentation to learn how to automate a specific Illumina library prep kit.

SPT Labtech

Beckman Coulter

Eppendorf

Hamilton

Revvity

Tecan

Related resources

How to choose a LIMS for NGS

See how you can benefit from a LIMS optimized for next-generation sequencing. Find out what features to look for when choosing a LIMS for NGS.

Multiplex sequencing strategies

Learn about sample multiplexing strategies that allow large numbers of libraries to be pooled and sequenced simultaneously during a single run.

Need more info?

Contact us to learn more about automation partners and Illumina Qualified methods.