Library Prep Automation

Automation Methods that Span Our Library Prep Portfolio

Minimize errors, reduce hands-on time, and enable higher throughput

Automate Your Library Prep

Automated liquid handling systems help laboratories obtain and maintain consistent results while driving efficiency. Automation also allows for the rapid scaling of throughput without the need for additional hands-on time.

Automation partners

Illumina has partnered with leading automation vendors to help provide automated methods for our library prep kits on their flagship systems. Combining their automation knowledge with our library prep and sequencing expertise helps create a valuable solution for NGS configured automated systems.

Illumina Qualified methods

Illumina Qualified methods are available for most of our library prep portfolio. These methods are developed, tested, distributed, owned, and supported by our partners. Illumina reviews data to ensure method performance and data quality.

Vendor developed methods

Vendor developed methods are those that have been developed by a partner, but have not undergone data review by Illumina. These methods are included on the chart below, but do not have the Illumina Qualified identifier.

Library Prep Automation Partner Chart

Please reference the chart and associated legend below to determine which methods are Illumina Qualified.

To view partner documentation, please click on the partner name at the top of the chart.

 Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)

  Agilent Beckman Coulter Eppendorf Hamilton Revvity Tecan
Illumina Stranded Total RNA   Biomek i7 epMotion 5075t NGS STAR Sciclone DreamPrep
Illumina Stranded mRNA   Biomek i7 epMotion 5075t NGS STAR Sciclone DreamPrep
Illumina RNA Prep with Enrichment Bravo Option A Biomek i7 epMotion 5075t NGS STAR Sciclone DreamPrep
Illumina DNA PCR Free   Biomek i5
Biomek i7
epMotion 5075t NGS STAR Sciclone
Zephyr
DreamPrep
Freedom EVO NGS
Illumina DNA Prep Bravo Option B
Bravo Option A
Biomek FXp
Biomek iSeries
NGeniuS
epMotion 5075t NGS STAR Sciclone DreamPrep
Freedom EVO NGS
Illumina DNA Prep with Enrichment Bravo Option B
Bravo Option A
Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone DreamPrep
Freedom EVO NGS
Nextera XT DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone DreamPrep
Freedom EVO NGS
AmpliSeq for Illumina*   Biomek i5 epMotion 5075t
epMotion 5073
NGS STAR
NGS STARlet
Sciclone  
TruSight Oncology 500HT   Biomek i7   NGS STAR with 96 MPH/SBS STAR MOA   DreamPrep
TruSight Oncology 500   Biomek NXp
Biomek i5
  NGS STAR/SBS STAR LSA
NGS STARlet
Sciclone DreamPrep
TruSight Tumor 15   Biomek 4000 epMotion 5075t   Zephyr  
TruSight Tumor 170   Biomek NXp   NGS STAR    
TruSeq DNA PCR-Free Bravo Option B Biomek FXp epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq Nano DNA Bravo Option B Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq Stranded mRNA Bravo Option B
Bravo Option A
Biomek FXp
Biomek iSeries
epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS
TruSeq Stranded Total RNA Bravo Option B Biomek FXp epMotion 5075t NGS STAR Sciclone NGS and NGSx Freedom EVO NGS

Starting in January 2022 Hamilton will refer to the NGS STAR configuration for TruSight Oncology library preparations as the SBS STAR LSA. All existing Illumina Qualified methods are backwards compatible and will continue to run on this system as this change is in name only.

*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.

† 500 High-throughput (HT)

Need additional information?

Contact our Automation Specialists to learn more about automation partners and Illumina Qualified methods.

Contact Us

Automated Library Prep Workflow Examples

Assessing Nucleic Acid Quality with the Agilent Fragment Analyzer System

The Fragment Analyzer accurately assesses quality and quantity of isolated nucleic acids and prepared libraries to optimize cluster generation during sequencing. System options accommodate both low- and high-throughput labs.

Download
Automated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics

The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.

Download
TSO 500 high-throughput automation learn more
Automating TruSight Oncology 500 High-Throughput

Learn more about automated methods from Beckman Coulter and Hamilton for streamlined, consistent library prep.

Read Hamilton Tech Note
Read Beckman Coulter Tech Note
Illumina high-throughput sequencing

Adopt High-Throughput Sequencing In Your Lab

High-throughput sequencing can cost-effectively run data-rich applications and process more samples to improve statistical signficance. Get rapid access to more data for greater insights.

Learn More

Library Prep Technical Tips

DNA/RNA Isolation Considerations

Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.

dsDNA Library Concentration

Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.

Quantification and Quality Control

Find out how to quantify and validate final libraries for a successful sequencing run.