Automate Your Library Prep
Automating library preparation can help increase your lab's efficiency and generate more consistent results. Automation can also help you maintain turnaround times, despite expanding sample workloads. To aid with adopting NGS automation, methods for many of our library prep assays are available from the leading automation companies. Our partners offer a range of automation platforms to address your laboratory's throughput needs.
Both Illumina and our partners have created methods to address the increasing range of automation products and library prep. Methods created by a partner are tested, distributed, and typically supported by that partner. Illumina-developed methods are distributed by Illumina and are usually supported by our partners.
Illumina Qualified Methods are available for several of our library prep kits. We verify that libraries created with these methods perform comparably to manually prepared libraries. These methods are developed by partners who test for functional performance and provide distribution and support.
We make it easy to automate your library prep. The vendors below have methods designed for Illumina kits, eliminating the time and effort needed to develop your own method.
| Agilent | Beckman Coulter | Eppendorf | Hamilton | PerkinElmer | Tecan | |
|---|---|---|---|---|---|---|
| Illumina Stranded Total RNA | epMotion 5075t | STAR NGS | ||||
| Illumina Stranded mRNA | epMotion 5075t | STAR NGS | ||||
| Illumina RNA Prep with Enrichment | Biomek i7 | epMotion 5075t | Sciclone | |||
| Illumina DNA PCR Free | Biomek i5 Biomek i7 |
epMotion 5075t | NGS STAR | Sciclone Janus |
Fluent | |
| Illumina DNA Prep | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Sciclone | Freedom EVO NGS |
| Illumina DNA Prep with Enrichment | Bravo Option B | Biomek iSeries | epMotion 5075t | NGS STAR | Scicione | Freedom EVO NGS |
| Nextera XT DNA | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Scicione | Freedom EVO NGS |
| AmpliSeq for Illumina* | Biomek i5 | epMotion 5075t epMotion 5073 |
NGS STAR NGS STARlet |
Scicione | ||
| TruSight Oncology 500 | Biomek FXp Biomek i5 |
NGS STAR | ||||
| TruSight Tumor 15 | Biomek 4000 | epMotion 5075t | Zephyr | |||
| TruSight Tumor 170 | Biomek NXp | NGS STAR | ||||
| TruSeq DNA PCR-Free | Bravo Option B | Biomek FXp | epMotion 5075t | NGS STAR | Scicione NGS and NGSx | Freedom EVO NGS |
| TruSeq Nano DNA | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Scicione NGS and NGSx | Freedom EVO NGS |
| TruSeq RNA Exome | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Scicione NGS and NGSx | Freedom EVO NGS |
| TruSeq RNA Fusion | epMotion 5075t | |||||
| TruSeq RNA Pan Cancer | ||||||
| TruSeq RNA v2 | Biomek FXp | Scicione NGS and NGSx | ||||
| TruSeq Stranded mRNA | Bravo Option B | Biomek FXp Biomek iSeries |
epMotion 5075t | NGS STAR | Scicione NGS and NGSx | Freedom EVO NGS |
| TruSeq Stranded Total RNA | Bravo Option B | Biomek FXp | epMotion 5075t | NGS STAR | Scicione NGS and NGSx | Freedom EVO NGS |
| 16S rRNA | epMotion 5075t epMotion 5073 |
NGS STAR |
Illumina Qualified (Illumina analysis has shown that libraries prepared with these methods perform comparably to those prepared manually)
Documentation: user guide and / or app note available
*In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for Illumina kit may be required when preparing the maximum number of libraries per kit.
Frequently Purchased Together
Automated Library Prep Workflow Examples
Assessing Nucleic Acid Quality with Fragment Analyzer™
The Fragment Analyzer offers automated fragmentation and size selection. It accurately assesses quality and quantity of prepared libraries to optimize cluster generation during sequencing, and it accommodates both low- and high-throughput labs.
DownloadAutomated Nextera DNA Flex Library Prep (now Illumina DNA Prep) Workflow for High-Throughput Metagenomics
The streamlined, automated workflow generates highly uniform libraries and provides excellent data for species identification, metagenomic profiling, and de novo genome assembly.
DownloadLibrary Prep Technical Tips
DNA/RNA Isolation Considerations
Find guidance to help you avoid contamination while purifying DNA/RNA before library preparation.
dsDNA Library Concentration
Learn how to convert library concentration from ng/µl to nM for some Illumina library preparation methods.
Quantification and Quality Control
Find out how to quantify and validate final libraries for a successful sequencing run.