NGS Experimental Design
Designing Your Experimental Protocol
These recommendations and best practices are designed to help you ensure the success of your next-generation sequencing (NGS) experiment. Review key considerations, such as calculating read length, estimating coverage, and measuring sequencing accuracy. Use our interactive tools to help you create an NGS protocol or select the right products and methods for your project.
Jump-Start Your NGS Experiment
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Contact Us View Data SheetOther NGS Experimental Considerations
Library Quantification
How to Achieve Consistent Quantitation Part 1
In NGS experiments, library quantification can affect the quality of your sequencing run.
How to Achieve Consistent Quantitation Part 2
Find tips and tricks for obtaining reliable, consistent quantitation.
Library Quantification and QC Reference Guide
Learn how to quantify and validate libraries for a successful sequencing run.
Sequencing Quality
Optimizing Cluster Density
The most common cause of over- and under-clustering is inaccurate library quantification.
Nucleotide Diversity
The presence of diverse or balanced libraries is important for generating high-quality sequencing data, especially in the first few cycles.
Quality Monitoring
PhiX is a concentrated Illumina library that can serve as a calibration control for NGS experiments.
Planning an Experimental Budget
As next-generation sequencing costs continue to decline, Illumina is leading the way in making NGS more affordable and accessible. With these resources, we’ll guide you through key factors to consider when planning your NGS budget.
View NGS Cost ConsiderationsExperiment Planning Tools
Find interactive tools designed to help you choose the right methods and products for your needs.
View Tools
Additional Resources
NGS Tutorials
Find tutorials designed to help you understand key concepts in NGS.
Sequencing Data Analysis
Find intuitive data analysis solutions that allow you to spend more time doing research and less time setting up workflows.
Technical Bulletins
Explore experimental design tips and resources in our repository of technical support bulletins and reference material.
Online Training
Access technical support training courses, webinars, and video tips.