Information about instrument components and software, performing a sequencing run, and maintenance.
Preparing a lab for the NextSeq 550Dx instrument: lab space, electrical, bench requirements, and user-supplied consumables.
Online Illumina sequencing courses are free, interactive, and available any time.
This tool will help you determine the best hands-on sequencing training for your needs based on your area of interest, application, library preparation kit, and platform.
Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.
Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.
User guide, safety guide, site preparation guide, and related application and tech notes for the NextSeq 550Dx instrument.
These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantitation.
United States and Canada:
The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx instrument is to be used with specific registered and listed, cleared or approved, IVD reagents and analytical software.
European Union and Rest of World:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved in vitro diagnostic reagents and analytical software.