Education and resources for sequencing on the NextSeq 550Dx

Familiarize yourself with and get started on the NextSeq 550Dx instrument

NextSeqDx System Resources

Find tools to help you choose the right products for your needs, and plan your experiments.

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Product Selection and Experiment Planning Tools
NextSeq 550Dx Instrument Reference Guide

Information about instrument components and software, performing a sequencing run, and maintenance.

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NextSeq 550Dx Instrument Site Prep Guide

Preparing a lab for the NextSeq 550Dx instrument: lab space, electrical, bench requirements, and user-supplied consumables.

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Sequencing Online Training

Online Illumina sequencing courses are free, interactive, and available any time.

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Personalized Training Workflow Selection Tool

This tool will help you determine the best hands-on sequencing training for your needs based on your area of interest, application, library preparation kit, and platform.

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Sequencing Coverage

Sequencing coverage describes the average number of NGS reads that align to known reference bases. Coverage requirements vary by application.

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Quality Scores

Additional information about sequencing quality scores, how they are calculated, and the relationship between quality score and base call accuracy.

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NextSeq 550Dx Documentation

User guide, safety guide, site preparation guide, and related application and tech notes for the NextSeq 550Dx instrument.

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Sequencing Troubleshooting Tips

These short videos provide expert tips for common sequencing issues such as overclustering and inconsistent quantitation.

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Intended Use for the NextSeq 550Dx

United States:
The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. The NextSeq 550Dx instrument is to be used with registered and listed, cleared or approved, IVD reagents and analytical software.

European Union:
The NextSeq 550Dx instrument is intended for sequencing of DNA libraries when used with in vitro diagnostic (IVD) assays performed on the instrument. The NextSeq 550Dx instrument is to be used with specific registered, certified or approved IVD reagents and analytical software.