NovaSeq X Series

Change the world with the NovaSeq X Series

Breakthrough innovations for groundbreaking discoveries

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The NovaSeq X System: Now shipping
Cost-effective, high-throughput sequencing is now even more accessible. Learn how

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Maximize throughput to unleash your ambitions

Extraordinary throughput and accuracy to perform data-intensive applications at production scale

Built on decades of innovation from a trusted partner in genomics, the NovaSeq X Series will enable your most ambitious ultra-high-throughput projects. These are our most powerful sequencers yet. Scale your studies with three flow cell types and 16–21 Tb output per run on the dual flow cell NovaSeq X Plus System or 8–10.5 Tb on the single flow cell NovaSeq X System.* Ultra-high-density flow cells and ultra-high-resolution optics enable output of 26–35 billion single reads per flow cell.

*Performance at the higher range of output specification is not guaranteed. Actual output is dependent on library type, user optimization, and run performance.

The NovaSeq X Series is powered by XLEAP-SBS chemistry — an even faster, higher quality, and more robust version of our proven sequencing by synthesis (SBS) chemistry

XLEAP-SBS chemistry delivers the highest level of data accuracy and performance, and the increased stability enables remarkable sustainability benefits.

Flexibility to run DRAGEN analysis your way, either onboard, in the cloud, or on-server

Access a broader menu of applications through cloud workflows on Illumina Connected Analytics or BaseSpace Sequence Hub.

The innovations built into the NovaSeq X and NovaSeq X Plus Systems have the power to generate tens of thousands of whole genomes per year.

Dream big. NovaSeq X Series can make it happen.

NovaSeq X Series specifications

Output range^a
~165 Gb–21 Tb
Reads per run
~1.6–52 billion
Max read length
2 × 300 bp
Run time
~17–48 hr

View all NovaSeq X specs

Transformative economics for transformative breakthroughs

The NovaSeq X Series allows you to maximize your productivity and minimize your turnaround time.

Beyond the significant reduction in cost per Gb and per read, the NovaSeq X series incorporates cost-efficiency throughout the workflow. Streamlined informatics, operational simplicity, sustainability advancements, and world-class support lead to the best total cost of ownership equation.

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The ultimate user experience

Push-button operation with automated onboard cluster generation and post-run wash
Individually addressable flow cell lanes with automated onboard independent lane loading
Flexible run planning options, touchless secondary analysis workflows, and automated lossless data compression
Thoughtful ergonomic design innovations for the ultimate user experience

Sequence more sustainably than ever

Propel your research forward to impact the world, not the environment. The NovaSeq X and NovaSeq X Plus Systems were purpose-designed to be our most sustainable sequencing systems yet.

Save time and money with a 90% reduction in packaging weight and waste
Maximize storage space with compact cartridges and packaging
Save disposal costs with no dry ice or ice packs

A new standard in operational simplicity

More results, fewer steps, less effort. The NovaSeq X Series workflow includes flexible run setup, automated ultra-high-throughput sequencing with onboard cluster generation, accelerated data analysis with DRAGEN platform onboard, and more.

Manage workflow

Track samples and manage your runs with manual or automated LIMS options.

Prepare libraries

Access a broad range of high-performance library preparation kits.

Sequence

Use XLEAP-SBS chemistry—advanced reagents built upon proven Illumina SBS—to power faster, more accurate sequencing.

Analyze data

Leverage DRAGEN platform onboard or in the cloud for accurate, rapid, secondary data analysis.

Interpret results

Interpret results, get insights, and drive discovery with cloud connection to tertiary analysis tools.

Unimaginable experiments, made unbelievably cost-efficient

The NovaSeq X Series helps moonshot projects become a reality—making affordable what was previously out of reach. Increase statistical power with broader study designs and larger sample cohorts. Maximize read numbers and increase sequencing depth for a higher resolution view that can detect low-frequency signals and variants.

The NovaSeq X Plus System revolutionizes human whole-genome sequencing, with the ability to sequence more than 128 genomes per run. These production-scale sequencing systems can also perform a broad range of data-intensive applications, from single-cell analysis to exome and circulating tumor DNA sequencing, that will unlock the next wave of omics discovery.

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Compare with NovaSeq 6000

NovaSeq X Plus^a

NovaSeq X^a

NovaSeq 6000

Maximum output per run

~16–21 Tb

~8–10.5 Tb

6 Tb

Maximum reads per run

52–70 billion single reads
104–140 billion paired-end reads

26–35 billion single reads
52–70 billion paired-end reads

20 billion single reads
40 billion paired-end reads

See all Illumina sequencers

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Want to learn more about instrument financing options?

Get up and running quickly with a rental or leasing program designed to work with your budget.

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Get help deciding if the flexibility and throughput of the NovaSeq X Series are right for you. Contact us today for more information.