NovaSeq X Applications & Methods
Applications & Methods
Unimaginable experiments, made unbelievably cost-efficient
The NovaSeq X Series offers vast application breadth, enabling data-intensive methods at production scale
Key Applications and Methods
The unmatched combination of production-scale throughput and cost enables deeper sequencing of larger sample cohorts. The NovaSeq X Plus system delivers up to 16 Tb of output, allowing sequencing of 128 human genomes at 30× coverage or 96 human genomes at 40× coverage per dual 25B flow cell run.
Illumina Complete Long Reads enables generation of both long- and short-reads on the same instrument for more scalable, comprehensive whole-genome sequencing.
Prep
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for applications such as whole-genome sequencing or tumor-normal sequencing.
Illumina Complete Long Read Prep
Illumina Complete Long Read Prep, Human, is designed for human whole-genome sequencing (WGS).
Sequence
NovaSeq X Series Reagent Kits
The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.
The 25B flow cell unlocks access to the highest throughput sequencing run on an Illumina sequencer. With 8 individually addressable lanes, and 2.5x greater output per run than the NovaSeq 6000 System, the 25B unlocks unprecedented sequencing scale and significant multiplexing to enable the deepest, broadest, and most cost-effective sequencing projects.
Analyze
DRAGEN Germline Pipeline
Full genome analysis with increased accuracy for variant calling, including repeat expansion detection and genotyping.
DRAGEN Illumina Complete Long Read WGS
Generates our most accurate and comprehensive whole human genome data using DRAGEN and Illumina Complete Long Reads.
Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq X Plus system offers sequencing of ~1500 exomes in a single run using a dual 25B flow cell run.
Prep
Illumina DNA Prep with Enrichment
This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
Sequence
NovaSeq X Series Reagent Kits
The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.
Analyze
DRAGEN Enrichment Pipeline
Exome analysis for variant detection, including a full suite of enrichment metrics and reporting.
Analyze coding plus multiple forms of noncoding RNA for a comprehensive picture of the transcriptome. With the NovaSeq X Plus system, you can sequence over 1000 transcriptomes in a single run using a dual 25B flow cell run.
Prep
Illumina Stranded Total RNA Prep
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
Sequence
NovaSeq X Series Reagent Kits
The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.
Unlock the next wave of genomic discovery
Increased discovery power will come from larger studies, deeper sequencing to identify rare genetic events, and broader sequencing methods and multiomics for a more comprehensive view of cellular activity. Learn how you can perform broader, deeper sequencing and take on projects that were previously out of reach with the NovaSeq X Series.
Download eBookMore Applications and Methods
Methylation Sequencing
Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.
Metagenomic Sequencing
Sequence thousands of organisms in parallel. Identify unculturable or low-abundance microbes, or evaluate microbial diversity.
mRNA Sequencing
Select and sequence polyadenylated transcripts for gene expression profiling studies.
High-Intensity Sequencing
Improve statistical power, get multidimensional insights, and increase analytical resolution with cost-efficient high-throughput sequencing workflows.
Tumor-Normal Sequencing
Genome-wide comparison of tumor vs matched normal DNA to inform analysis of oncogenes, tumor suppressors, and other risk factors.
Single-Cell RNA Seq
Assess the individual contributions of single cells in complex tissues by profiling the transcriptome.
ATAC-Seq
The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome.
ChIP-Seq
Chromatin immunoprecipitation sequencing (ChIP-Seq) is a powerful method for analyzing DNA-protein interactions and performing genome-wide surveys of gene regulation.
De Novo Sequencing
De novo sequencing refers to sequencing a novel genome with no reference sequence available. NGS enables fast, accurate characterization of any species.
Target Enrichment
Capture genomic regions of interest via hybridization to target-specific biotinylated probes.
Long Read Sequencing
A highly accurate approach that can help resolve challenging regions of the genome such as complex structural variants and highly repetitive elements.