Illumina sequencing technology, sequencing by synthesis (SBS), is a widely adopted next-generation sequencing (NGS) technology worldwide, responsible for generating more than 90% of the world's sequencing data.1 Illumina sequencing instruments and reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.
A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all 4 reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias.
The end result is true base-by-base sequencing that enables accurate data for a broad range of applications. The method virtually eliminates errors and missed calls associated with strings of repeated nucleotides (homopolymers).
Illumina sequencing by synthesis technology supports both single-read and paired-end libraries. SBS technology offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, structural variation detection, and more.
The combination of short inserts and longer reads increases the ability to fully characterize any genome.
Find out how 2-channel SBS, a recent evolution of SBS technology, enables faster sequencing with the same high data accuracy.
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1. Data calculations on file. Illumina, Inc. 2015.
See SBS technology in action.
Explore a variety of methods enabled by SBS, from whole-genome to RNA sequencing and more. Find comprehensive workflow solutions.
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The NextSeq Series enables everyday genome, exome, transcriptome sequencing, and more.
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NextSeq 500 v2 Kits offer better data quality, an improved signal to noise ratio, and a simpler workflow compared to the prior v1 kit.
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This overview describes major sequencing technology advances, key methods, the basics of Illumina sequencing chemistry, and more.
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See how patterned flow cell technology dramatically increases sequencing data throughput.
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