Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

This kit removes both cytoplasmic and mitochondrial rRNA from human, mouse, and rat samples, including partially degraded samples. Read More...
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Ribo-Zero Gold rRNA Removal Kit (H/M/R) (6 reactions)

MRZG126

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Ribo-Zero Gold rRNA Removal Kit (H/M/R) (24 reactions)

MRZG12324

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Product Highlights

  • Removes ribosomal RNA (rRNA) in a single pass
  • Sequencing data contains complete transcriptome of coding and noncoding RNA species
  • Good for intact and partially degraded small sample quantities – only 1 μg of total RNA required

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with Ribo-Zero Gold (Human/Mouse/Rat) is focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

Ribo-Zero Gold (Human/Mouse/Rat) offers informative sequencing results by removing unwanted rRNA prior to sequencing. This kit removes both cytoplasmic (nuclear-encoded) and mitochondrial rRNAs.

Ribo-Zero retains the complete transcriptome

Ribo-Zero removes rRNA in order to retain the regulatory regions of noncoding RNA, even from low-quality samples. Other methods, such as Poly(A) depletion, do not retain noncoding, regulatory information.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted from intact, degraded, and badly degraded (FFPE) samples in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kits

Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat): Removes cytoplasmic rRNA only.

ScriptSeq Complete Gold Kit (Human/Mouse/Rat): Compatible with low input samples (e.g. 100 ng of total RNA).

Related information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
Ribo-Zero Kit FAQs

Customer Stories

BRC-Seq Customers Benefit from Streamlined BaseSpace Integrated Workflow

BaseSpace enables tracking of samples, from library prep to data analysis, helping BRC-Seq deliver high-quality sequencing data and additional value to its customers.

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Supporting Data and Figures

Generate RNA-Seq libraries enriched for coding and noncoding content
Generate RNA-Seq libraries enriched for coding and noncoding content

Total RNA samples treated with Ribo-Zero (H/M/R) or Ribo-Zero Gold (H/M/R) generate RNA-Seq libraries enriched for coding and noncoding content. Total RNA from MCF-7 cells was treated with either the Ribo-Zero (H/M/R) Kit or the Ribo-Zero Gold (H/M/R) Kit. RNA-Seq libraries were prepared with the ScriptSeq v2 Kit and sequenced on an Illumina HiSeq 2000. Data courtesy of Vladimir Benes and Jonathon Blake, EMBL GeneCore, Heidelberg, Germany.

 

Product Literature

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