Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (to be discontinued)

This kit removes unwanted ribosomal RNA from Gram-positive bacterial samples.Read More...
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Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) 6 reactions


Product Highlights

  • Removes ribosomal RNA (rRNA) from intact and partially degraded Gram (+) bacteria
  • Sequencing data contains complete transcriptome of coding and noncoding RNA species
  • Good for small sample quantities – only 1 μg of total RNA required
  • Nuclear rRNA removed: 23S, 16S, 5S

Sequencing is an important discovery tool for research into disease, development, and more. The sequencing data from samples treated with the Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) offers informative sequencing results by removing unwanted rRNA prior to sequencing. The kit removes rRNA from both intact and partially degraded samples.

Removes all major rRNA subunits

The Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) depletes unwanted rRNA from total RNA. Removal of these unwanted subunits produces sequencing results that are heavily focused on biologically informative genomic regions.

Overview of the Ribo-Zero process

Virtually all rRNA is depleted in 2 hours.

Step 1: Wash the magnetic beads
Step 2: Treat samples with rRNA Removal Solution
Step 3: Remove rRNA
Step 4: Purify rRNA-depleted samples, and proceed with library preparation

Alternative kit versions

The Ribo-Zero rRNA Removal Kit (Bacteria) is recommended for samples composed of mixtures of Gram-negative and Gram-positive bacteria.

Related information

rRNA Removal Kit Selection Guide
Ribo-Zero Kit Species Compatibility
RNA Matchmaker Tool (test rRNA removal kit compatibility in silico with your species of interest)
Ribo-Zero Kit FAQs

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Product Literature

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