Products for the NextSeq Series

Kits, reagents, and clinical research products compatible with the NextSeq 500 and NextSeq 550 desktop sequencing systems

NextSeq Products

Areas of Research Focus

Product Categories

Nucleic Acid Type

Key Use

Technology
Library Preparation Kits
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Nextera DNA Library Preparation Kit

Nextera DNA kits provide a fast and easy library prep workflow, delivering whole-genome sequencing libraries in under 90 min.

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Nextera Rapid Capture Custom Enrichment Kit

Custom target enrichment kit to rapidly interrogate those portions of the human genome most important to your specific research.

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Nextera Rapid Capture Exome and Expanded Exome Kits

Identify coding variants up to 70% faster with this exome library prep and enrichment kit. Expanded kit sequences untranslated regions (UTRs) and miRNA.

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Nextera XT DNA Library Preparation Kit

Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in less than 90 minutes, with a low DNA input requirement.

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SureCell WTA 3′ Library Prep Kit for the ddSEQ System

A robust, scalable, and user-friendly workflow for transcriptome profiling of hundreds to tens of thousands of single cells in a given experiment.

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TruSeq ChIP Library Preparation Kit

Simple, cost-effective chromatin immunoprecipitation sequencing (ChIP-Seq) DNA library preparation, with master mixes and robust multiplex capabilities.

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TruSeq Custom Amplicon Low Input Kit

This library prep kit delivers sensitive and specific amplicon sequencing results from both low-input and FFPE DNA samples.

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TruSeq Custom Amplicon v1.5

Focus on key genomic regions of interest with this targeted resequencing solution, useful for very high plexity pools or longer amplicons.

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TruSeq DNA Methylation Kit

Converts bisulfite-treated, single-stranded DNA into an Illumina sequencing library. Enables comprehensive whole-genome DNA methylation analysis.

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TruSeq DNA PCR-Free Library Preparation Kit

Simple, all-inclusive whole-genome sequencing (WGS) library preparation that provides accurate and comprehensive coverage of complex genomes.

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TruSeq Exome

This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.

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TruSeq Methyl Capture EPIC Library Prep Kit

This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylation’s role in gene regulation.

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TruSeq Nano DNA Library Prep Kit

Generate whole-genome sequencing libraries and efficiently interrogate samples with limited available DNA.

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TruSeq Nano DNA Library Prep Kit for NeoPrep

DNA library preparation for use with the NeoPrep System, providing comprehensive genomic coverage with low DNA input and minimal hands-on time.

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TruSeq RNA Access Library Prep Kit

Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.

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TruSeq RNA Library Prep Kit v2

The TruSeq RNA Library Preparation Kits provide a simple, cost-effective solution for analysis of the coding transcriptome.

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TruSeq Rapid Exome Library Prep Kit

This exome sequencing library prep kit uses a simple, one-day workflow to provide a fast path to greater understanding of the human exome.

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TruSeq Ribo Profile

The TruSeq Ribo Profile kit (formerly ARTseq) sequences ribosome-protected mRNA fragments to provide a snapshot of the active ribosomes in a cell.

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TruSeq Small RNA Library Preparation Kits

These kits provide a simple, cost-effective solution for generating miRNA and small RNA sequencing libraries directly from total RNA, for any species.

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TruSeq Stranded Total RNA Library Prep Kit

A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and FFPE.

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TruSeq Stranded Total RNA with Ribo-Zero Globin

Prepare whole-transcriptome sequencing libraries from blood-derived RNA with an efficient workflow that removes rRNA and globin mRNA in a single step.

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TruSeq Stranded Total RNA with Ribo-Zero Plant

Capture a comprehensive view of the plant transcriptome with this RNA-Seq library prep kit. Remove rRNA from plant leaf, seed, and root tissue.

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TruSeq Stranded mRNA Library Prep Kit

Prepare sequencing libraries from mRNA to get a clear view of the coding transcriptome with strand-specific information.

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TruSeq Stranded mRNA Library Prep Kit for NeoPrep

Complete view of the transcriptome with walk-away workflow, lower input requirement, and excellent reproducibility.

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TruSeq Targeted RNA Expression Apoptosis Panel

A targeted RNA sequencing panel for studying apoptosis, or programmed cell death. Assay targets pro-apoptotic and anti-apoptotic genes.

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TruSeq Targeted RNA Expression Cardiotoxicity Panel

A targeted RNA sequencing panel to study cellular pathways affected by cardiotoxic compounds or stress. Assay targets cardiotoxic response markers.

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TruSeq Targeted RNA Expression Cell Cycle Panel

A targeted RNA sequencing panel for studying the cell cycle. Assays target genes involved in all cell cycle phases as well as DNA replication.

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TruSeq Targeted RNA Expression Cytochrome p450 Panel

A targeted RNA sequencing panel for studying cytochrome p450 (CYP) genes. Assays target CYP genes involved in drug and toxin metabolism.

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TruSeq Targeted RNA Expression Hedgehog Panel

A targeted RNA sequencing panel for studying hedgehog signaling. Assays target upstream and downstream signal transduction and transcription factors.

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TruSeq Targeted RNA Expression Library Prep Kits

Targeted RNA assays for gene expression studies: fixed or custom sequencing panels, with a simple workflow and automated data analysis.

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TruSeq Targeted RNA Expression NFkB Panel

A targeted RNA sequencing panel for studying NFκB signaling. Assays target upstream and downstream signal transduction and NFκB transcription factors.

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TruSeq Targeted RNA Expression Neurodegeneration Panel

A targeted RNA sequencing panel for studying neurodegenerative and neurotoxic pathways such as those implicated in Alzheimer’s Disease.

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TruSeq Targeted RNA Expression Stem Cell Panel

A targeted RNA sequencing panel for studying various stem cell types. Analyze stem cell and pluripotency markers, cytokines, growth factors, and more.

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TruSeq Targeted RNA Expression Wnt Panel

A targeted RNA sequencing panel for studying Wnt signaling. Assays upstream and downstream signal transduction genes, transcription factors, and more.

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TruSeq Targeted RNA Expression p53 Panel

A targeted RNA sequencing panel for studying p53 signaling. Assays target genes involved in signal transduction, as well as transcription factors.

Cluster Generation and Sequencing Reagents
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NextSeq 500/550 v2 Kits

These sequencing reagent kits offer a simplified workflow and improved data quality compared to the prior v1 kit version.

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PhiX Control v3

PhiX Control v3 is a ready-to-use control library for Illumina sequencing runs.

Clinical Research Products
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TruSight Cancer

This expert-defined sequencing panel targets 94 genes and 284 SNPs associated with a predisposition towards various cancers.

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TruSight Cardio Sequencing Kit

This kit provides researchers with a comprehensive, cost-effective solution for identifying causal variants implicated in inherited cardiac conditions (ICCs).

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TruSight HLA v2 Sequencing Panel

Unambiguous, phase-resolved HLA typing in a single assay using proven Illumina NGS technology and Assign analysis software.

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TruSight Inherited Disease

TruSight Inherited Disease is a targeted sequencing panel that focuses on 552 genes associated with severe, recessive pediatric onset diseases.

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TruSight Myeloid Sequencing Panel

The TruSight Myeloid Sequencing Panel targets variants associated with myeloid malignancies such as AML, MDS, MPN, CML, CMML, and JMML.

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TruSight One Sequencing Panel

This panel focuses on exonic regions harboring disease-causing variants. Labs can perform commonly ordered molecular assays on-site with one assay.

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TruSight RNA Fusion Panel

Provides comprehensive gene fusion detection in formalin-fixed, paraffin-embedded (FFPE) and other cancer research samples.

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TruSight RNA Pan-Cancer

Targeting 1385 cancer genes for gene expression, variant, and fusion detection studies in many RNA sample types including blood, bone marrow, and FFPE.

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TruSight Rapid Capture

TruSight Rapid Capture all-in-one kits enable targeted sequencing library preparation and enrichment for up to 96 samples in only 1.5 days.

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TruSight Tumor 170

Comprehensive next-generation sequencing (NGS) assay that targets DNA and RNA variants from the same FFPE sample.

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TruSight Tumor 26

This targeted sequencing panel enables clinical researchers to identify low-frequency variation in solid tumors across 26 genes.

Molecular Biology Reagents
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Globin-Zero Gold rRNA Removal Kit

This kit removes unwanted ribosomal RNA and globin mRNA from mammalian blood RNA samples before sequencing.

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Ribo-Zero Gold rRNA Removal Kit (Epidemiology)

This kit removes cytoplasmic and mitochondrial rRNA from samples composed of human/mouse/rat, Gram-positive and Gram-negative bacterial RNA.

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Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat)

This kit removes both cytoplasmic and mitochondrial rRNA from human, mouse, and rat samples, including partially degraded samples.

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Ribo-Zero Gold rRNA Removal Kit (Yeast)

This kit removes both cytoplasmic and mitochondrial ribosomal RNA from yeast samples.

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Ribo-Zero rRNA Removal Kit (Bacteria)

This kit removes ribosomal RNA from Gram-positive and Gram-negative bacterial mixtures in a single pass.

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Ribo-Zero rRNA Removal Kit (Gram-Negative Bacteria)

This kit removes unwanted ribosomal RNA from Gram-negative bacterial samples.

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Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria)

This kit removes unwanted ribosomal RNA from Gram-positive bacterial samples.

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Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat)

This kit removes cytoplasmic ribosomal RNA from human, mouse, or rat samples, including partially degraded samples.

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Ribo-Zero rRNA Removal Kit (Plant)

This kit removes cytoplasmic, mitochondrial, and chloroplast ribosomal RNA from plant seed, root, or leaf samples in a single pass.