ScriptSeq Complete Gold Kit (Epidemiology)

This kit removes ribosomal RNA from mixtures of human/mouse/rat and bacterial samples in a single pass, and creates sequencing libraries. Read More...
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ScriptSeq Complete Gold Kit (Epidemiology) 6 Reactions

BEP1206

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ScriptSeq Complete Gold Kit (Epidemiology) 24 Reactions

BEP1224

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Accessory Products
What accessories do I need?

ScriptSeq Index PCR Primers (Set 1)

RSBC10948

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ScriptSeq Index PCR Primers (Set 2)

SSIP1202

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ScriptSeq Index PCR Primers (Set 3)

SSIP1203

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ScriptSeq Index PCR Primers (Set 4)

SSIP1204

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ScriptSeq Index PCR Primers (Sets 1-4)

SSIP1234

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Product Highlights

  • Remove ribosomal RNA (rRNA) from intact and partially degraded mixtures of human/mouse/rat and bacteria in a single pass
  • Ideal for host/pathogen studies
  • Create a stranded Illumina sequencing library
  • Sequencing data contain complete transcriptome of coding and non-coding RNA species
  • Barcode/index-compatible: ScriptSeq index primers available separately for preparing barcoded libraries

Sequencing is an important discovery tool for disease studies. The sequencing data from samples treated with ScriptSeq Complete Gold (Epidemiology) are focused on valuable RNA species, to facilitate discovery of relevant genes, splice variants, and isoforms.

The ScriptSeq Complete Gold Kit (Epidemiology) offers the most informative sequencing results by removing unwanted rRNA before creation of a stranded sequencing library. The kit efficiently removes rRNA from samples composed of human/mouse/rat and bacteria. When performing stranded analysis on samples prepared with the ScriptSeq Complete Gold Kit (Epidemiology), choose the FR=secondstrand option.

Related Information

Specifications

Supporting Data and Figures

 

rRNA Types Removed

ScriptSeq Complete Gold Kit (Epidemiology) depletes rRNA from human/mouse/rat and bacteria
Sample Input Type RNA Input Quantity from Human/Mouse/Rat and Bacteria Removes Gram (+) and Gram (-) Bacterial rRNA 23S, 16S, 5S Removes Human, Mouse or Rat mt rRNA 16S, 12S Removes Human, Mouse or Rat Cytoplasmic rRNA 28S, 18S, 5.8S, 5S
Standard input 500 ng – 2.5 μg total RNA

Product Literature

Literature is not currently available for this product.

The low input versions of this kit (catalog numbers SCL6EP and SCL24EP) have been discontinued. The standard kit versions can process low input RNA with a minor protocol modification.